Detection ability of nucleic acid screen laboratory in blood bank based on NCCL external quality assessment data
10.13303/j.cjbt.issn.1004-549x.2022.02.022
- VernacularTitle:基于NCCL室间质评数据的血站核酸检测实验室检测能力分析
- Author:
Yuena XIE
1
;
Tong PAN
1
;
Miao LIU
1
;
Fengyuan LI
1
;
Jun LIU
1
Author Information
1. Tianjin Blood Center, Tianjin 300110, China
- Publication Type:Journal Article
- Keywords:
external quality assessment (EQA);
blood banks;
nucleic acid test(NAT)
- From:
Chinese Journal of Blood Transfusion
2022;35(2):212-215
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To evaluate the laboratory's NAT ability by analyzing the feedback reports of nucleic acid test (NAT) results of external quality assessment (EQA) of National Center for Clinic Laboratories (NCCL), so as to improve the laboratory management details and ensure blood safety. 【Methods】 The data of NCCL NAT EQA of blood screening laboratory of Tianjin Blood Center (a total of five occasions from Jan 2019 to Jun 2021) were statistically analyzed. 【Results】 From Jan 2019 to Jun 2021, the laboratory participated in EQA for five times and all the results were qualified. The test results of NAT EQA HIV RNA/HCV RNA/HBV DNA detected by R1, R2 and R4 were consistent with the reference results. R3 showed false positive results (CT value 40.46) in the single donation detection of sample No.1925 in HCV RNA. Unreported data of the laboratory was that in the first EQA in 2021, the R4 showed false positive results (CT value 35.8) in in the single donation detection of sample No.2113 in HIV RNA. 【Conclusion】 The performance of each NAT screening system in our laboratory is relatively stable except occasional false positive results influenced by every factor. Potential problems can be found and continuously improved by assaying EQA reports and the extended experimental results of EQA samples to further improve the detection ability.
