Optimization of detection conditions of pre-donation ABO blood type screening by sliding methods in fixed blood donation sites
10.13303/j.cjbt.issn.1004-549x.2022.02.008
- VernacularTitle:街头采血纸板法ABO血型检测条件优化探讨
- Author:
Dan LI
1
;
Yonglei LYU
1
;
Lili FENG
1
;
Xu WANG
1
;
Xiaotian ZHANG
1
;
Yajuan LIU
1
;
Panpan XU
1
;
Lei ZHAO
1
Author Information
1. Henan Red Cross Blood Center, Zhengzhou 450052, China
- Publication Type:Journal Article
- Keywords:
fixed blood donation sites;
ABO blood group initial screening;
sliding methods;
blood safety
- From:
Chinese Journal of Blood Transfusion
2022;35(2):161-163
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the quantitative value of key points for ABO blood group initial screening in fixed blood donation sites, so as to provide reference for standardized testing process of sliding method. 【Methods】 Several groups of experiments were carried out to illustrate the optimal conditions, including the serum dosage of monoclonal reagent, red blood cell dosage of blood sample, and reaction time in ABO blood group initial screening, by sliding method, and the quantitative value of key points of sliding method was preliminarily determined. Blood typing tests of 310 blood donor samples including type A, B, O, AB, subtype A and subtype AB were conducted to evaluate the effects of quantitative value of key points in the initial screening procedure. The test tube method would be conducted if the results are inconsistent with the fully automated blood grouping analyzer. The ABO subtypes suspected are identified by serological and molecular biological methods. 【Results】 The quantitative value of key points in initial screening procedure of sliding methods was as follows: 2 drops of reagent serum, 5-10 μL of whole blood and 3 minutes of reaction time. The concordance rate of ABO blood type screening comparison experiment in 310 blood donors was 100%. 【Conclusion】 ABO blood group initial screening by sliding method with quantitative value can effectively standardize the pre-donation blood type screening in fixed blood donation sites, and can meet the requirements of ABO blood group initial screening.
