Frequency of JK (a-b-) in Yichang: genetic pedigree analysis of Jk (a-b-) and Jka weak expression
10.13303/j.cjbt.issn.1004-549x.2022.02.006
- VernacularTitle:宜昌地区献血者Jk(a－b－)频率与Jka弱表达表型分子遗传机制研究
- Author:
Juncui GAO
1
;
Yurong YUAN
1
;
Yuxiang LIN
2
,
3
;
Haojun ZHANG
4
;
Shangen ZHENG
5
Author Information
1. Yichang Red Cross Central Blood Station, Yichang 443005, China
2. Medical School and the First Affiliated Hospital of Xi′an Jiaotong University
3. Baorui Biotechnology Taizhou Co. Ltd.
4. Baorui Biotechnology Taizhou Co. Ltd.
5. Central Theater General Hospital
- Publication Type:Journal Article
- Keywords:
Jk(a-b-);
Jkaw+;
pedigree analysis;
Kidd blood group;
SLC14A1 gene
- From:
Chinese Journal of Blood Transfusion
2022;35(2):154-156
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the molecular hereditary and frequency of Jk(a-b-) in blood donors in Yichang. 【Methods】 A total of 49 999 samples from Yichang Red Cross Central Blood Station were screened for Jk(a-b-) by urea hemolysis test(2 mol /L). The phenotypes of JK (a-b -) probands and their families were confirmed by monoclonal anti-Jka and anti-Jkb, and the whole exon of SLC14A1 gene was sequenced. 【Results】 The frequency of Jk(a-b-) in Yichang blood donors was 0.004% (2/49 999), and the exon sequencing of SLC14A1 gene confirmed that both two probands were JK*02N.01 caused by c. 342-1G>A homozygous mutation.Besides, JK*01W.01 allele was observed in the pedigree analysis, and weak expression of Jka was found in 4 out of 11 family members. 【Conclusion】 The frequency of JK (a-b -) in Yichang blood donors is similar to those in Shanghai 0.004%(2/48 400), and both caused by JK * 02N.01 allele with high frequency in Southeast Asia. The epidemiological survey of JK * 01w.01 allele frequency should be further performed.
