The polymorphism of HPA-1-6w, HPA-15 and HPA-32bw-35bw in platelet donors from Deyang, Sichuan
10.13303/j.cjbt.issn.1004-549x.2022.02.005
- VernacularTitle:四川德阳地区血小板捐献者HPA-1～6w、HPA-15及HPA-32bw～35bw多态性研究
- Author:
Qilu LYU
1
,
2
;
Xiuyun LIAO
3
;
Xin JI
1
,
2
;
Li YUAN
3
;
Liqiong QIAN
3
;
Shichun SHU
3
;
Qiang CHEN
1
,
2
;
Jue WANG
1
,
2
Author Information
1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu 610025, China
2. Key Laboratory of Adverse Reactions of Blood Transfusion, Chinese Academy of Medical Sciences
3. Deyang Central Blood Station
- Publication Type:Journal Article
- Keywords:
human platelet antigen;
polymorphism;
platelet donors;
Deyang;
PCR-SBT
- From:
Chinese Journal of Blood Transfusion
2022;35(2):149-153
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the polymorphism of HPA-1-6w, HPA-15 and 32bw-35bw in platelet donors in Deyang, Sichuan, and estimate whether to include the detection of 32bw-35bw in the platelet bank. 【Methods】 Polymerase chain reaction with sequenced based typing (PCR-SBT) was used to sequence the HPA-1-6w, HPA-15 and 32bw-35bw loci of 205 platelet donors in Deyang. Allele frequencies were calculated by the direct counting method. The frequencies of HPA-1-6 and 15 alleles in northern and southern Chinese, Japanese and Australian population were compared, and those HPA loci and HPA-32bw-35bw were searched in the Chinese Millionome Database (CMDB) and genomAD to obtain the polymorphism data. Then the Chi-square test was performed with the data of this study through GraphPad Prism 9 software. 【Results】 The allele frequencies of HPA-1b, 2b, 3b, 5b, 6bw and HPA-15b were 0.005(2/410), 0.037(15/410), 0.471(193/410), 0.020(8/410), 0.010(4/410) and 0.461(189/410), respectively, b allele of HPA-32bw-35bw and HPA-4 was not detected. Statistical significance was observed between the HPA-1b allele frequency of this study and northern Chinese, Australian population and genomAD global population sample (P< 0.05, 0.005 vs 0.014 vs 0.145 vs 0.122). The frequency of HPA-2b alleles in this study, Japanese population and genomAD global population samples was 0.037 vs 0.120 vs 0.100, with statistical difference(P<0.05). Comparison of HPA-5b and HPA-6bw allele frequencies with those of genomAD global population showed a statistical difference (P<0.05, 0.020 vs 0.089 and 0.010 vs 0.000 008, respectively). 【Conclusion】 The polymorphisms of HPA-1-6w and HPA-15 of donors in Deyang has characteristics of the southern Chinese. The frequencies of HPA-32bw-35bw were extremely low, which could be excluded from the platelet bank in Deyang.
