Preparation and preliminary identification of specific monoclonal antibody against AB blood group antigen
10.13303/j.cjbt.issn.1004-549x.2021.10.026
- VernacularTitle:特异性抗-AB血型抗原的单克隆抗体制备及初步鉴定
- Author:
Huan LIU
1
;
Shengbao DUAN
2
;
Hongmei WANG
2
;
Jing CHEN
3
;
Yong LI
2
Author Information
1. Suzhou Guoke Sibeta Biotechnology Co., Ltd., Suzhou 215163, China
2. Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences
3. Suzhou Blood Center
- Publication Type:Journal Article
- Keywords:
AB red blood cell;
anti-AB McAb;
preliminary identification
- From:
Chinese Journal of Blood Transfusion
2021;34(10):1156-1159
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To prepare and preliminarily identify the specific monoclonal antibody (McAb) against-AB blood group antigen. 【Methods】 The human AB red blood cells were used to immunize BALB/c mice for producing monoclonal antibodies against human red cell AB antigens. The cell lines that can secrete AB monoclonal antibodies were obtained by cell fusion and screening using hybridoma cell technology.The specificity and recognized epitopes of anti-AB McAb were preliminarily identified by blood group serological assay. The antibody was also used to detect the clinical samples. 【Results】 One hybridoma cell line secreting IgM monoclonal antibody against human AB antigens was obtained. This anti-AB McAb could agglutinate with human red blood cells of type A, B and AB, but not agglutinate with type O red blood cells. The red blood cell absorption and elution test confirmed that the antibody recognized the common epitope of A and B. The anti-AB McAb obtained and the commercial anti-A and anti-B reagents were used to detect 567 clinical samples in parallel, with the concordance rate at 100%. 【Conclusion】 A hybridoma cell line secreting anti-AB McAb can be successfully prepared.
