Identification of a rare anti-Dib combined with multiple antibodies and clinical blood transfusion practice
10.13303/j.cjbt.issn.1004-549x.2021.10.025
- VernacularTitle:罕见抗-Dib联合多种抗体的鉴定与临床输血实践
- Author:
Xiaoyue CHU
1
;
Juan MAO
1
;
Baoyan WANG
2
;
Qinqin ZUO
1
;
Dazhou WU
1
;
Weiwei ZHANG
1
;
Liangzi ZHANG
1
;
Hong WANG
1
;
Hua XU
1
Author Information
1. Shaanxi Blood Center, Xi′an 710061, China
2. Department of Blood Transfusion, the First Affiliated Hospital of Xi′an Jiaotong University
- Publication Type:Journal Article
- Keywords:
Diego blood group;
anti-Dib;
complex antibody;
absorption and elution test;
immune control
- From:
Chinese Journal of Blood Transfusion
2021;34(10):1152-1155
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To identify one blood sample with ambiguous antibody screening result, explore the identification strategy of complex antibodies and provide blood transfusion plan. 【Methods】 Blood typing and antibody screening were performed by serological test. The genotyping of Diego blood group was carried out by gene detection. Absorption and elution test were designed to identify the complex antibodies. 【Results】 Serological test showed that the patient′s blood type was B, ccDEE, Jk(a-b+ ), Le(a-b+ ), Fy(a+ b-), Di(a+ b-). The results of genotyping showed that the Diego genotype of the patient was Di (a+ b-). Based on the above results, absorption and elution tests were designed and IgG anti-Dib, IgG anti-Ce, and IgG anti-Jka antibodies were detected in serum of the patient. Combined with the use of immunoglobulin and targeted blood transfusion measures, successful treatment was provided for the patient. 【Conclusion】 For the identification of antibodies against high frequency antigen combined with multiple antibodies, a variety of experimental techniques and methods should be combined, and the experimental scheme should be designed flexibly.
