Epitope analysis and molecular study of weak D type 136: A case report
10.13303/j.cjbt.issn.1004-549x.2021.10.024
- VernacularTitle:弱D136型血型抗原表位分析及分子基础研究
- Author:
Zhen WANG
1
;
Shuangshuang JIA
1
;
Jizhi WEN
1
;
Hong LUO
1
;
Runqing ZHANG
1
;
Yanli JI
1
;
Guangping LUO
1
Author Information
1. Institute of Clinical Transfusion, Guangzhou Blood Center, Guangzhou 510095, China
- Publication Type:Journal Article
- Keywords:
Rh blood group;
D variant;
weak D;
epitopes of RhD antigen
- From:
Chinese Journal of Blood Transfusion
2021;34(10):1149-1152
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To identify the blood group epitope of a D variant individual and analyze its molecular characteristics. 【Methods】 The saline test and indirect antiglobulin test (IAT) were used to identify the RhD serologically. The anti-human globulin gel card was used for direct antiglobulin test (DAT). RhD epitopes were detected using the epitope detection kit (D-Screen). RhCE antigens were typed using Rh typing Card. The RHD gene zygomorphism was further analyzed by PCR-RFLP. Ten exons of RHD gene were amplified by PCR and analyzed by direct sequencing. 【Results】 DAT test was negative, and the serological results showed weak expression of RhD, which was D variant. The RhD epitope test results showed that the red blood cells of this patient had a weak agglutination with 4 monoclonal anti-D against epD6.4, epD6.1, epD2.1, and epD5.4 (w+ to 2+ ), and reacted negatively with other epitope antibodies. RhCE antigen typing was Ccee; The RHD gene zygomorphism result was D+ /D-, the sequencing of RHD exons revealed that the first exon carried c. 41C>T (p.Pro14Leu) missense mutation, and its genotype was RHD*01W.136/01N.01. 【Conclusion】 This D variant is the first weak D type 136 reported in the Chinese population, and its phenotype is weak partial D.
