Effect of HLA-G expressed in platelets on Tax protein of human T lymphocyte leukemia type 1 virus
10.13303/j.cjbt.issn.1004-549x.2021.10.002
- VernacularTitle:血小板表达的HLA-G对人类T淋巴细胞白血病1型病毒蛋白Tax表达的影响
- Author:
Shaojia ZHANG
1
,
2
;
Guizeng ZHAO
3
;
Hang DONG
1
;
Mengyao LI
1
;
Xiaojia YU
4
;
Lixiang WANG
1
;
Chenguang ZHANG
1
Author Information
1. School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, China
2. The Fourth Clinical College of Xinxiang Medical University
3. The First Clinical College of Xinxiang Medical University
4. The Third Clinical College of Xinxiang Medical University
- Publication Type:Journal Article
- Keywords:
human leukocyte antigen G;
human T-lymphocyte leukemia type 1 virus;
platelet;
immune regulation
- From:
Chinese Journal of Blood Transfusion
2021;34(10):1066-1069
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effect of HLA-G expressed in platelets on Tax protein of human T cell leukemia type 1 virus (HTLV-1). 【Methods】 Platelets were isolated from anticoagulant whole blood, and HLA-G molecule on platelet membrane was detected by flow cytometry. The content of secretory HLA-G before and after platelet lysis was detected by ELISA, HTLV-1 human lymphoma cells MT2 were cultured with platelet lysate (PL). The effect of HLA-G in platelets on the expression of HTLV-1 protein Tax was evaluated by Western blot (WB). 【Results】 Membrane type mHLA-G was highly expressed on the surface of platelet membrane. The expression of secretory sHLA-G (ng/mL) increased after platelet lysis (15.73±1.01) vs (6.65±0.47), the expression of sHLA-G increased with the increase of platelet concentration in a dose-dependent manner. Compared with fetal bovine serum, PL significantly promoted the high expression of HLA-G protein and HTLV-1 virus tax protein in MT2 cells, and the addition of anti-HLA-G antibody to PL could effectively inhibit the expression of Tax and HLA-G protein. 【Conclusion】 High expression of immune tolerance molecule HLA-G on platelets can induce high expression of HTLV-1 protein Tax in human lymphoma cell MT2, which contributes to viral infection.
