Method for the detection of human immunoglobulin Fc function based on surface plasmon resonance: preliminary establishment and validation
- VernacularTitle:基于表面等离子共振法的人免疫球蛋白Fc段活性检测方法的初步建立与验证
- Author:
Fangyu DONG
1
;
Jiuyue ZHOU
1
;
Chen CHEN
1
;
An ZHOU
1
;
Dianwei SONG
1
;
Jianfeng GAO
1
;
Xi CHEN
1
;
Xiao LI
1
;
Jiawei WU
2
;
Jiacheng DU
2
;
Bo ZHOU
1
;
Changyong JIAN
1
Author Information
- Publication Type:Journal Article
- Keywords: immunoglobulin; surface plasmon resonance; Fc segment; molecular interaction analyzer
- From: Chinese Journal of Blood Transfusion 2022;35(4):396-399
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To establish a method for detecting human immunoglobulin Fc function based on surface plasmon resonance technology. 【Methods】 Based on the characteristic that FcγRI can be binded to the Fc segment of IgG, the affinity constant of the sample was detected by surface plasmon resonance, and its Fc function was the KD ratio of the sample to the standard. The method was validated for specificity/specificity, precision and robustness. The method and the pharmacopoeia method were used to detect the Fc function of 30 human immunoglobulins, and the correlation and consistency of the detection results were analyzed. 【Results】 The method validation results showed that this method has strong specificity/specificity (t values were 0.15, 0.22, both P>0.05), good precision (CV value 5.37%~10.69%) and good robustness (CV value 10.06%). The detection results of this method and the pharmacopoeia method have high correlation (r=0.96, P<0.05) and high consistency (Bias-2.060, 95% Limits of Agreement-5.628~1.508). 【Conclusion】 A method for detecting human immunoglobulin Fc function based on surface plasmon resonance has been successfully established.
