Study on the repair effect of lyophilized platelet lysate products on articular cartilage injury model of rat
10.13303/j.cjbt.issn.1004-549x.2022.04.009
- VernacularTitle:冻干血小板裂解液制品对大鼠关节软骨损伤模型的修复作用
- Author:
Guangya LIU
1
;
Yubing XU
1
;
Yu ZHANG
1
;
Zhanhong ZHU
1
;
Mou ZHOU
1
;
Wei ZHANG
1
;
Guiqiu SHAN
1
Author Information
1. Department of Blood Transfusion, General Hospital of Southern Theater Command, Guangzhou 510010, China
- Publication Type:Journal Article
- Keywords:
platelet lysates;
lyophilized;
articular cartilage injury;
animal model;
SD rats;
repair of cartilage injury
- From:
Chinese Journal of Blood Transfusion
2022;35(4):392-395
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To discuss the repair effect of lyophilized platelet lysate (PL) products on articular cartilage injury model of rats. 【Methods】 A total of 25 SD rats were injected with typeⅡcollagenase at the right knuckle articular cavity respectively on day 1, 3 and 5 of experiment, and the modeling conditions were observed 14 days after the last injection of collagenase. The SD rats with successful modeling were randomly divided into three groups, and were injected with lyophilized PL [Group A, 1 mL/(mouse·time)], PL [Group B, 1 mL/(mouse·time)], and normal saline[Group C, 1 mL/(mouse·time)]. The above three substances were injected with corresponding drugs on day 0, 7, 14 and 21 of experiment based on the grouping conditions, and the changes of knee joint diameters of the rats from the three groups were observed and compared. On day 14 and 28, one rat in each group was randomly killed and two knuckle articular cavities of each were taken for tissue sampling, using hematoxylin-eosin staining (HE) and immunohistochemistry. 【Results】 After 14 days of modeling by injection of type Ⅱ collagenase, the proportion of successful modeling in rats was 84% (21/25), with the knee joint diameter (mm) before and after modeling at 12.84±1.14 vs 14.11±1.17(P<0.01). On day 14, 21 and 28, groups A and B were superior to group C in the knee joint diameter and activity improvement (P<0.05), with 13.33±1.16 vs 13.37±1.08 vs 14.21±1.08, 13.10±1.09 vs 13.01±1.04 vs 14.09±1.09 and 12.38±1.08 vs 12.51±1.03 vs 14.01±1.07, respectively. Histological observation showed that group A and B were superior to group C in the production and arrangement of chondrocytes and the positive expression of type Ⅱ collagen, and there was no significant difference between group A and group B. 【Conclusion】 The lyophilized PL has similar therapeutic effect to PL in the treatment and repair of articular cartilage injury, and is worthy of clinical application.
