Effect of Qingkailing injection on platelets function preserved in vitro
10.13303/j.cjbt.issn.1004-549x.2022.04.008
- VernacularTitle:清开灵注射液延长血小板体外保存期对其功能影响的研究
- Author:
Jie LI
1
;
Xuerui ZHANG
1
;
Wenbin FENG
1
;
Yan HUANG
2
;
Jun WANG
1
;
Yunlong WANG
1
;
Xiaoxia TAN
1
;
Jiongcai LAN
3
Author Information
1. Department of Transfusion Medicine, Beihai People’s Hospital, Beihai 536000, China
2. Beihai Blood Center
3. Nanfang Hospital, Southern Medical University
- Publication Type:Journal Article
- Keywords:
Qingkailing injection;
platelet preservation in vitro;
platelet function;
platelet activity
- From:
Chinese Journal of Blood Transfusion
2022;35(4):387-391
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effect of Qingkailing injection on platelet function preserved in vitro. 【Methods】 A total of 15 bags of plateletpheresis (≥250 mL/bag), adding Qingkailing injection(1.25 mL/bag) at the final concentration of 1%, were stored at 22 ℃ with gentle agitation as the experimental group, and samples were collected on day 1, 3, 5, 8, 10 and 14 to detect the thromboelastogram (TEG), CD62p expression rate and mitochondrial membrane potential (JC-1). The control group was set up synchronously, with the same volume and storage conditions as the experimental groups, and samples were taken on day 1, 3 and 5 after preservation to undertake the same test items as the experimental groups. The differences of detection indexes between the two groups were compared. 【Results】 1) In the experimental group, there was no significant change in K and α during day 1 to 14(P>0.05). The R value (min) increased from 6.12±1.58 to 11.02±2.26, and the CI value changed from 0.27±1.24 to -10.47±3.51 (P<0.05). The MA value (mm) had no significant change within 5 days (P>0.05), but decreased to 53.18±2.71 on day 8 and 22.88±3.45 on day 14 (P<0.05). In the control group during day 1 to 5, K(min) and α showed no significant change, while R(min) was significantly prolonged, MA (mm) decreased and CI increased significantly (P<0.05). 2) The expression rate of CD62p (%) was 70.50±9.12 in the experimental group on day 5 (vs 83.16±5.33 in the control groups, P<0.05) and 82.77±7.17 on day 14 (P<0.05). 3) There was no significant change in JC-1 (%) during regular preservation period between the experimental group and control group (P>0.05), but JC-1 (%) was 86.75±3.88 vs 70.36±19.8 on day 5 (P<0.05). In the experimental group, JC-1(%) was 81.04±9.50 vs 71.38±8.77 vs 82.77±7.17 on day 8, 10 and 14, respectively. 【Conclusion】 The activation and aggregation as well as anti-apoptosis function of plateletpheresis, adding Qingkailing injection at the final concentration of 1%, are similar to those of routine preservation.
