Establishment of CD36 negative platelet donor bank in Zhongshan area

Yonglun WU; Ainong Sun; Fei PU; Qiao LI; Yuru Fang; Qianying Chen; Yanting Liao; Hongmei Wang; Yezhou Chen; Shengbao Duan

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Establishment of CD36 negative platelet donor bank in Zhongshan area

VernacularTitle:中山地区CD36阴性血小板供者库建立的探讨
Author: Yonglun WU ¹ ; Ainong SUN ¹ ; Fei PU ¹ ; Qiao LI ¹ ; Yuru FANG ¹ ; Qianying CHEN ¹ ; Yanting LIAO ¹ ; Hongmei WANG ^{2
,

3} ; Yezhou CHEN ^{2
,

3} ; Shengbao DUAN ^{2
,

3}
Author Information

1. Zhongshan Blood Center, Zhongshan 528400, China
2. Suzhou Institute of Biomedical Engineering and Technology
3. Jihua Laboratory
Publication Type:Journal Article
Keywords: CD36 antigen; platelet; blood donors; donor bank
From: Chinese Journal of Blood Transfusion 2022;35(5):558-561
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the frequency of CD36 deletion and gene mutation in voluntary blood donors of Zhongshan city, and to explore the possibility of establishing local CD36 negative platelet donor bank. 【Methods】 Platelet CD36 antigen was detected by ELISA in 1 654 voluntary blood donors.Some of the negative samples were confirmed by flow cytometry, and genotyping was also performed. 【Results】 Platelet CD36 antigen was negative in 27 cases, accounting for 1.6% (27/1654), among which 1.6% (18/1149) were males and 1.8% (9/505) were females.No significant difference was noticed between males and females in CD36 antigen deletion cases (P>0.05). Fifteen CD36 negative samples were randomly selected, genotyped and sequenced, with type I deletion in 1 case[ 6.7% (1/15)], type Ⅱ deletion in 14 cases[ 93.3% (14/15)], and gene mutation in exon 3-14 detected in 8 cases. 【Conclusion】 The frequency of platelet CD36 antigen deletion in Zhongshan is comparable to that in other southern regions of China.The establishment of CD36 negative platelet donor bank is conductive to improve the effectiveness of platelet transfusion.