CMV-IgG yeild in Guangzhou blood donors and comparison of different test reagents
10.13303/j.cjbt.issn.1004-549x.2022.05.017
- VernacularTitle:广州献血人群CMV-IgG阳性率及不同检测试剂的比较研究
- Author:
Zhengang SHAN
1
;
Qiao LIAO
1
;
Ru XU
1
;
Min WANG
1
;
Jieting HUANG
1
;
Huishan ZHONG
1
;
Yongshui FU
1
;
Xia RONG
1
Author Information
1. Guangzhou Blood Center, Guangzhou 510095, China
- Publication Type:Journal Article
- Keywords:
blood donors;
cytomegalovirus;
antibody positive rate
- From:
Chinese Journal of Blood Transfusion
2022;35(5):549-552
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the CMV-IgG positive yeild among blood donors in Guangzhou and explore the differences in the efficacy of three test reagents, aimed at improving blood safety and service capacity of blood centers. 【Methods】 A total of 630 blood samples from eligible blood donors from July to October 2020 in our center were randomly selected and screened for CMV-IgG by one ELISA reagent.Among them, 180 samples were tested in parallel using three reagents (two ELISA reagents and one ECLIA reagent), and those tested negative were conducted quantitative CMV-DNA detection.The test results of different reagents were compared and analyzed. 【Results】 Out of the 630 samples, a total of 598 positive samples were screened out, including 180 samples yielded by three reagents, 171 and 175 by the two ELISA reagents, respectively, and 175 by ECLIA.The results given by three reagents were consistent (Kappa>0.4), and no significant difference in the positive yeild by three reagents was found.In the 180 samples, 11 were negative, among which 3, 2 and 6 samples were negative by all three reagents, two reagents and one reagent (ELISA), respectively.All the 11 samples were tested negative for CMV-DNA. 【Conclusion】 The yeild of positive CMV-IgG in blood donors was 94.9% (598/630), suggesting a high prevalence of CMV in Guangzhou. CMV serologically negative blood should be considered when providing blood products to immunocompromised patients to improve the safety of recipients.The detection results of ELISA reagents and ECLIA reagent for CMV- IgG are consistent, but ECLIA reagent has better detection efficacy.
