Exploration on the preparation conditions of platelets as samples for biomarker detection
10.13303/j.cjbt.issn.1004-549x.2022.05.009
- VernacularTitle:血小板作为生物标志物检测标本的制备条件探索
- Author:
Caoyi LIU
1
;
Zhou LI
2
;
Yang GAO
1
;
Yu LIU
1
Author Information
1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China
2. Transfusion Department, Affiliated Children′s Hospital, Capital Institute of Paediatrics
- Publication Type:Journal Article
- Keywords:
platelet preparation;
platelet protective solution;
preparation conditions;
platelet activation rate
- From:
Chinese Journal of Blood Transfusion
2022;35(5):514-519
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To optimize the preparation conditions of platelets as biomarkers to ensure the preparation of platelet samples with low activation and good stability. 【Methods】 The platelet count, platelet activation rate and the count of residual leukocytes were compared among the control and four experimental groups with different centrifugation conditions (120g 10 min vs. 120g 15 min vs.120g 20 min vs.240g 20 min)and different formulations of protective solution, i.e.apyrase, dipyridamole, cilostazol and L-arginine (experimental group 1) vs.apyrase, dipyridamole, cilostazol and adenosine (experimental group 2) vs.apyrase, cilostazol, L-Arginine and adenosine (experimental group 3) vs. apyrase, dipyridamole, L-arginine and adenosine (experimental group 4). 【Results】 Platelets obtained by centrifugation at 120 g for 15 min(73.88±5.36) and centrifugation at 120 g for 10 min(77.65±3.07) had higher recovery rates than other experimental groups(52.77±7.86 and 37.71±13.82)(P<0.05). They also had lower activation rates than other experimental groups, but the differences were not statistically significant(P>0.05). The residual leukocytes in the experimental group centrifuged at 120g for 15 min was slightly lower than that in the experimental group centrifuged at 120 g for 10 min, with no statistical differences (P>0.05). The platelet activation rates of four experimental groups were all significantly lower than that of the control within 72 hours (P<0.05). The platelet activation rate within 24~72 hours was lower in experimental group 1 than the other three experimental groups, with significant difference versus experimental group 2 and experimental group 3 (P<0.05), but not versus experimental group 4 (P>0.05). 【Conclusion】 For preparing platelets as biomarker samples, the group with solution including apyrase, dipyridamole, cilostazol and L-arginine, and centrifugation at 120 g for 15min, showed the minimum residual leukocytes and the optimum stability within 72 hours.
