Genetic analysis of a weak D type61 sample from a blood donor, Jiangyin

Fang Wang; Mengyao Bian; Qiurong YU; Minglei WU; Haiping Zhao; Ling Sun; Buqiang Wang; Hongjun Gao; Haicai Shi; Yi WU; Ming Gao; Yuping Chen

Return

Genetic analysis of a weak D type61 sample from a blood donor, Jiangyin

VernacularTitle:无偿献血者弱D61标本基因分析
Author: Fang WANG ¹ ; Mengyao BIAN ² ; Qiurong YU ¹ ; Minglei WU ³ ; Haiping ZHAO ¹ ; Ling SUN ³ ; Buqiang WANG ² ; Hongjun GAO ³ ; Haicai SHI ³ ; Yi WU ³ ; Ming GAO ² ; Yuping CHEN ²
Author Information

1. Jiangyin Branch Station, Wuxi Central Blood Station, Jiangyin 214400, China
2. Jiangsu Libo Medicine Biotechnology Co., Ltd
3. Jiangsu Zhongjiwat Biotechnology Medicine Co., Ltd
Publication Type:Journal Article
Keywords: Rh blood type; D^el; weak D61; genetic analysis
From: Chinese Journal of Blood Transfusion 2022;35(7):701-704
CountryChina
Language:Chinese
Abstract: 【Objective】 To genetically analyze the D^el sample from a blood donor in Jiangyin and make clear the molecular basis of the serological phenotype. 【Methods】 The EDTA anticoagulant blood were collected: buffy coat were used for nucleic acid extract and cDNA analysis; red blood cells for serological test. Tube method and microcolumn gel were used for serological test. Genotyping kit were used for exon analysis. Gene mutation was analyzed using the sequence analyzer. 【Results】 Serological analysis demonstrated the sample′s RhD phenotype was D^el. The phenotype of RhCE was CCEe. Real-time fluorescence quota PCR result demonstrated the existence of all exones. Weak D15 and RHD^* DEL1 [RHD(1227G>A)], which had a high frequency of occurrence in China, were excluded according to real-time fluorescence quota PCR result. Sequence analyzing result verified RHD(28C>T) SNP mutation in cDNA. The genotype of this sample was RHD^*01 W. 61[RHD(28C>T)]. 【Conclusion】 A weak D61 was found among blood donors in our city, Jiangyin.