Identification and pedigree investigation of B(A) and cisAB blood groups
10.13303/j.cjbt.issn.1004-549x.2022.07.004
- VernacularTitle:B(A)与cisAB血型的鉴定分析及家系调查
- Author:
Yunqing SHEN
1
;
Jing LI
1
;
Yingfang PAN
1
;
Na ZHOU
1
;
Qun XU
1
Author Information
1. Blood Center of Shandong Province, Jinan 250014, China
- Publication Type:Journal Article
- Keywords:
cisAB;
B(A);
blood type;
serology;
genotyping;
pedigree investigation
- From:
Chinese Journal of Blood Transfusion
2022;35(7):697-701
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the serological and molecular genetic characteristics of B(A) and cisAB blood groups discovered in our laboratory. 【Methods】 ABO blood group serology and genetic tests were used to identify blood groups of 6 blood samples, submitted by blood center and hospitals in Shandong, and pedigree investigation was carried on 2 of them. 【Results】 Among the 6 samples, serological results were B(A) in 5 cases and cisAB in 1 case. The results of genetic tests were consistent with the serological results, as the alleles included B(A)04, B(A)02 and cisAB01, and all genotypes were heterozygous with O. Serological pedigree study was conducted on 2 samples: One cisAB patient with his 4 relatives(cisAB type father and three O type relatives) and one B(A)02/O1 donor with his 3 relatives[ B(A) type father/brother and O type mother). For B(A)02/O1 donor, the results of genetic testing were consistent with the serological results, as the paternal genotype was the same as that of the proband, the younger brother was B(A)02/O2, and the maternal genotype was O1/O2. 【Conclusion】 The cisAB and B(A) blood groups are often indistinguishable by serological phenotypes and require genetic confirmation. CisAB pedigree investigation revealed 2 cases of cisAB blood type and B(A) pedigree investigation revealed 3 cases of B(A). The genotyping of cisAB and B(A) in this region were cisAB01/O2, B(A)02/O1, B(A)02/O2, B(A)04/O1 and B(A)04/O2. B(A)and cisAB subtypes can be accurately identified through genetic testing and pedigree investigation, which can provide a reliable basis for blood transfusion selection and ensure the safety of clinical blood transfusion.
