The establishment of a high-resolution melting curve genotyping method for ABCG2*376T allele of Jr(a-) and its distribution frequency study
10.13303/j.cjbt.issn.1004-549x.2022.07.002
- VernacularTitle:Jr(a-)稀有血型ABCG2*376T等位基因高分辨熔解曲线检测方法的建立及分布频率研究
- Author:
Zhen WANG
1
;
Shuangshuang JIA
1
;
Zhijian LIAO
1
;
Chunyan MO
1
;
Ling WEI
1
;
Runqing ZHANG
1
;
Guangpin LUO
1
;
Yanli JI
1
Author Information
1. Institute of Clinical Transfusion, Guangzhou Blood Center, Guangzhou 510095, China
- Publication Type:Journal Article
- Keywords:
Jr(a-);
ABCG2*376T allele;
high-resolution melting curve;
distribution frequency
- From:
Chinese Journal of Blood Transfusion
2022;35(7):690-693
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To establish a high-throughput detection method for ABCG2*376T allele of Jr(a-), and apply it to the study of the frequency of this allele in the Chinese population. 【Methods】 The specific primers were designed and synthesized, the sample carrying homozygous ABCG2*376T alleles, obtained in the previous study, was used as the homozygous positive control, and the sample carrying heterozygous allele as the heterozygous positive control. The wild-type sample was used as a negative control, and a high-resolution melting curve(HRM) method for detecting this allele was established. The established method was used to screen DNA samples from blood donors in Guangzhou, and the samples carrying ABCG2*376T alleles were sequenced to confirm the accuracy of the HRM method. 【Results】 A HRM method, which can detect ABCG2*376T allele and accurately type homozygotes and heterozygotes at the same time, had been established successfully. Fifteen individuals with heterozygous alleles were screened out of 1 560 blood donors in Guangzhou, while none homozygous allele was detected. 【Conclusion】 The HRM method can be used to accurately screen and type ABCG2*376T allele. The frequency of this allele in Chinese population is about 0.48%(15/3120).
