Serological and molecular characterization of HBV infection with HBsAg reactive and HBV DNA non-reactive
10.13303/j.cjbt.issn.1004-549x.2022.09.016
- VernacularTitle:HBsAg＋&HBV DNA－的HBV感染的血清学和分子生物学特性
- Author:
Chunxiang LI
1
;
Xiaohan GUO
1
;
Peng SUN
1
;
Xiaochun LIU
1
;
Dan LIU
1
;
Lei ZHOU
1
;
Liang ZANG
1
;
Daniel CANDOTTI
2
,
3
;
Xuelian DENG
1
Author Information
1. Dalian Blood Center, Dalian 116001, China
2. Department of Virology, Henri Mondor Hospital, AP-HP
3. University of Paris-Est, INSERM U955, IMRB, Créteil, France
- Publication Type:Journal Article
- Keywords:
blood screening;
HBsAg positive;
nucleic acid testing;
HBV infection;
mechanisms
- From:
Chinese Journal of Blood Transfusion
2022;35(9):950-954
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the serological and molecular characteristics of HBsAg+ /HBV DNA non-reactive (NR) infections. 【Methods】 Samples tested as HBsAg+ and HBV DNA NR were confirmed by individual NAT repeat testing, viral particle concentration by PEG precipitation combined with in-house nested PCR and real-time quantitative PCR, anti-HBc testing, and HBsAg quantification. HBV sequences were compared with those from donors with chronic and occult infection as controls. 【Results】 A total of 792 195 samples were screened between January 2011 and December 2020, of which 53 (1: 14 947) were confirmed HBsAg+ /HBV DNA NR. HBV DNA was detected further in five (9.4%) samples; three S sequences and four Pre Core/Core sequences were obtained. Unique amino acid substitutions (P130T, P135Q/S, R151Q, G153S and S155F) were found in the Core protein that may affect virus packaging and replication. 【Conclusion】 Extremely low HBV DNA level was detected in plasmas of HBsAg+ /HBV DNA NR donors. Barely detectable HBV DNA might be associated with unusual mutations in the Pre Core/Core protein affecting viral replication. More sensitive HBV DNA and/or HBsAg assays may be considered to further reduce the potential HBV transfusion-transmission residual risk.
