HBV genotyping and population genetics analysis of voluntary blood donors in five regions of China
10.13303/j.cjbt.issn.1004-549x.2022.12.015
- VernacularTitle:中国5个地区无偿献血者HBV基因分型及群体遗传学分析
- Author:
Mengyi ZHAO
1
;
Heng WEN
1
;
Anqing LIU
1
;
Limin CHEN
1
;
Zhan GAO
1
;
Miao HE
1
Author Information
1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences&Peking Union Medical College, Chengdu 610052, China
- Publication Type:Journal Article
- Keywords:
HBV;
genotype;
voluntary blood donors;
population genetics
- From:
Chinese Journal of Blood Transfusion
2022;35(12):1251-1255
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the distribution of Hepatitis B virus(HBV)genotypes and the genetic characteristics of genotype B HBV populations among voluntary blood donors in five regions of China. 【Methods】 A total number of 630 plasma samples from blood donors with positive HBV HBsAg neutralization test from 2014 to 2016 in Guangxi Blood Center, Chongqing Blood Center, Urumqi Blood Center, Mianyang Central Blood Station and Luoyang Central Blood Station were collected. The S-region sequence of the HBV genome was amplified by semi-nested PCR and followed with Sanger sequencing in order to investigate the HBV genotype distribution and population genetics. 【Results】 Among the voluntary blood donors in five regions, 55 cases of HBV S gene fragments were successfully amplified. Three genotypes were found in HBV typing, including 46 cases of type B(83.64%), 7 cases of type C(12.73%) and 2 cases of type D(3.63%). There were 15 cases of type B and 3 cases of type C in Guangxi; 10 cases of type B and 1 case of type C in Chongqing; 3 cases of type B, 1 case of type C and 1 case of type D in Luoyang; 15 cases of type B in Mianyang; 3 cases of type B, 2 cases of type C and 1 case of type D in Urumqi. The mismatch distribution of the HBVB type population with the largest population number showed a unimodal distribution, and the results of Tajima′s D test and Fu′s Fs test were both negative, indicating that the HBV population in these five regions was expanding. 【Conclusion】 The prevalent genotype of HBV in voluntary blood donors is type B, and the type B HBV population is experiencing a slow expansion, which should attract our attention. In the future, a thorough molecular epidemiological investigation of HBV should be carried out to ensure blood safety.
