Effect of red blood cell preservation solution on serum agglutination reaction of specific patients
10.13303/j.cjbt.issn.1004-549x.2022.11.021
- VernacularTitle:红细胞保存液对特定患者血清凝集反应的影响
- Author:
Xiao HAO
1
;
Lining MA
1
;
Huanan LIU
2
;
Xiaoye XIA
1
;
Xueqin DIAO
1
;
Xuejing LI
1
Author Information
1. Jinan Blood Station, Jinan 250001, China
2. Jinan Fourth People′s Hospital, Jinan 250001, China
- Publication Type:Journal Article
- Keywords:
erythrocyte protective agent;
Cross matching;
inconsistent positive and negative typing
- From:
Chinese Journal of Blood Transfusion
2022;35(11):1174-1176
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the precautions of pre-transfusion examination in patients with antibodies to erythrocyte protective solution, discrepant ABO blood typing results, and positive unexpected antibodies, so as to ensure the safety of blood transfusion. 【Methods】 The screen cells were divided into two groups according to the presence or absence of washing reagent red blood cells in normal saline. One group had untreated forward typing cells, antibody screening cells and identification panel, and the other group had saline-washed reverse typing cells, antibody screening cells and identification panel. The experiments were carried out by microcolumn gel method, saline medium method and polyamine method to analyze the effect of red blood cell preservation solution on serum agglutination reaction of specific patients. 【Results】 Among the 8 patients, forward typing was AB (+ ) in 1 patient, B (+ ) in 4, and A(+ ) in 3, and the reverse typing were interfered. The plasma of 8 patients agglutinated with unwashed reverse typing cells (saline tube method), screen cells and identification panels (saline tube method plus cassette method), while not agglutinated with the polybrene method. The interference was eliminated as using washed reverse typing cells (salinetube method), screen cells and identification panels (saline tube method plus cassette method). 【Conclusion】 The erythrocyte preservation solution affected patients’ blood group typing, but not affected the outcome of blood transfusion as no adverse reactions occurred.
