Study on separation and purification process of C1 esterase inhibitor
10.13303/j.cjbt.issn.1004-549x.2022.11.007
- VernacularTitle:C1酯酶抑制剂分离纯化工艺研究
- Author:
Jiabin XU
1
;
Yawen LI
1
;
Jiali GONG
1
;
Erhua LUO
1
;
Meng ZHANG
1
Author Information
1. Boya Bio-pharmaceutical Group Co. Ltd.Fuzhou 344000, China
- Publication Type:Journal Article
- Keywords:
C1 esterase inhibitor;
hereditary angioedema (HAE);
polyethylene glycol 4000;
Fractogel EMD SE HiCap(M) gel;
human prothrombin complex (PCC)
- From:
Chinese Journal of Blood Transfusion
2022;35(11):1121-1124
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To study the technology of separating and purifying C1 esterase inhibitor (C1-INH) by using the waste washing liquid as raw materia during the preparation of human prothrombin complex (PCC) l. 【Methods】 C1-INH was isolated and purified by a two-step method of polyethylene glycol (PEG) 4000 precipitation and cation chromatography. The pH of raw materials and the concentration of PEG4000 were adjusted to investigate the optimal conditions of PEG4000 precipitation method. After PEG was precipitated and centrifuged, the supernatant is treated as the loading solution for cation exchange chromatography, using Fractogel EMD SE HiCap(M) gel and CM Sepharose FF gel for ion exchange chromatography. The most suitable gel and separation conditions were selected by comparing the C1-INH antigen yield, activity yield and specific activity. 【Results】 Under the condition of pH 6.1, when the mass fraction of PEG4000 was 14%, the recovery rate of C1 esterase inhibitor was close to 70%, and the removal rate of ceruloplasmin was more than 95% after stirring for 10 minutes. As fractogel EMD SE HiCap(M) gel was used for cation exchange chromatography, when the eluent salt concentration was 0.25 M sodium chloride, the activity yield of C1 esterase inhibitor was greater than 80%, and the specific activity was greater than 5 IU/mg. 【Conclusions】 Using the waste washing liquid as the raw material during the preparation of PCC, the C1 esterase inhibitor with high specific activity can be prepared through PEG precipitation and purification by Fractogel EMD SE HiCap(M) ion exchange chromatography.
