Effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1
10.13303/j.cjbt.issn.1004-549x.2022.11.005
- VernacularTitle:贮存时间对红细胞非典型趋化因子受体1清除及储存趋化因子的影响
- Author:
Shihang ZHOU
1
;
Ni WANG
1
;
Ming LIU
2
;
Xiaohua LIANG
1
Author Information
1. Dalian Blood Center, Dalian 116001, China
2. Department of Cell Biology, Dalian Medical University
- Publication Type:Journal Article
- Keywords:
ACKR1;
chemokine;
erythrocytes
- From:
Chinese Journal of Blood Transfusion
2022;35(11):1113-1116
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To determine the effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1(ACKR1). 【Methods】 Samples from six bags of red blood cells product, split into two gruoups(10 mL each), were stored in a refrigerator and sampled on day 5 and day 25 during storage, respectively, to measure the concentrations of CCL5, CXCL8 and CCL11 and the ACKR1 chemokine scavenging function of erythrocytes. In addition, 42 erythrocyte products(1 mL each), stored for 5 to 25 days, were sampled to measure the expression of ACKR1 and the concentrations of chemokines CCL5, CXCL8 and CCL11 on the erythrocyte membrane. 【Results】 Compared with RBCs stored for 5 days, no difference in the concentration of CCL5(467.7±250.2 pg/mL vs 586.9±209.5pg/mL, P>0.05) and CCL11(122.2±30.3pg/mL vs 125.5 ±32.7pg/mL, P>0.05)were noticed in erythrocyte lysates stored for 25 days, but the concentrations of CXCL8 decreased significantly(42.4±5.3pg/mL vs 24.3± 5.9pg/mL, P<0.05), and the scavenging ability of erythrocyte ACKR1 to chemokines CCL5(2 634.0±730.2pg/mL vs 453.8±257.4 pg/mL, P<0.05), CXCL8(1 117.0±236.6pg/mL vs 306.2±28.3pg/mL, P<0.05) and CCL11 decreased(1 278.0 ±164.5pg/mL vs 467.2 ±50.9pg/mL, P<0.05). Chemokines CCL5, CXCL8 and CCL11 had not been detected on the surface of erythrocyte membrane. There was no significant change in the expression of ACKR1 on the surface of erythrocytes from day 5 to day 25 (P>0.05). 【Conclusion】 Erythrocytes are the main places for storing ACKR1 binding chemokines. During the storage, the chemokine scavenging of erythrocyte ACKR1 and some intracellular the ACKR1 binding chemokines are reduced.
