Analysis of HLA-Ⅰ antibody specificity and estimation of antigen immunogenicity: 96 patients recieved genotype-matched platelet transfusions
10.13303/j.cjbt.issn.1004-549x.2022.11.003
- VernacularTitle:96例血小板基因配型患者的HLA-Ⅰ类抗体特性分析及抗原免疫原性估算
- Author:
Ying LIU
1
;
Xinyu HUANG
1
;
Bing ZHANG
1
;
Gang XU
1
;
Xianguo XU
1
;
Ji HE
1
;
Faming ZHU
1
Author Information
1. Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, China
- Publication Type:Journal Article
- Keywords:
platelet transfusion refractoriness;
HLA class Ⅰ antibody;
antigen mismatch possibility;
relative immunogenicity
- From:
Chinese Journal of Blood Transfusion
2022;35(11):1105-1109
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the specificity of HLA class-Ⅰ antibody in the patients received HLA-matched platelet transfusions and estimate the relative immunogenicity of HLA-Ⅰ antigens. 【Methods】 The samples from 96 patients who suffered from platelet transfusion refractorines(PTR) and applied for transfusion with genotype-matched platelet were collected. The specificity of HLA I antibody was detected by Luminex technique, and the antibody expression level was analyzed according to MFI. The mismatch rate of HLA antigen and relative immunogenicity of the population were estimated according to the allele frequency distribution of HLA-A and B loci as well as the yielding frequency of antibody. 【Results】 HLA-Ⅰ antibodies were detected in all 96 patients, with varied species of antibodies. The average positive yielding rates of antibodies corresponding to HLA-A, -B and -C magnetic bead coated antigens (97 in total) were 0.38, 0.47 and 0.28, respectively. Among the HLA-A and -B loci in the Zhejiang population, HLA-A2, A11, A24 and HLA-B60, B46, B58 were the antigens with higher frequency, and their relative immunogenicity was 0.403, 0.283, 0.342, and 0.100, 0.067, 0.178, respectively. 【Conclusion】 The specificity of HLA-Ⅰ antibodies in PTR patients is different, which confirms that the relative immunogenicity differs by HLA-A and -B antigens.
