Assessment of 3 enzyme linked immunosorbent assays and 1 pseudotype lentivirus-based neutralization test in detecting serum antibody in convalescent plasma from COVID-19
10.13303/j.cjbt.issn.1004-549x.2022.01.002
- VernacularTitle:不同ELISA试剂与1种假病毒中和试验检测COVID-19康复者恢复期血浆抗体结果分析
- Author:
Lilin WANG
1
;
Xuqun WU
1
;
Linfeng WU
1
;
Li NING
1
;
Liang LU
1
;
Jinhong LIU
1
;
Ran LI
1
;
Tong LI
1
;
Limin CHEN
2
;
Min XU
2
;
Jinfeng ZENG
1
Author Information
1. ShenzhenBlood Center, Shenzhen 518035, China
2. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Provincial key Laboratory for Transfusion Transmitted Diseases of Sichuan Province, Chengdu 610052, China
- Publication Type:Journal Article
- Keywords:
SARS-CoV-2 antibody;
convalescent plasma;
enzyme linked immunosorbent assay;
pseudotype lentivirus-based neutralization test;
detection rate;
IgG antibody;
IgM antibody
- From:
Chinese Journal of Blood Transfusion
2022;35(1):5-9
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To assess three severe acute respiratorysyndrome coronavirus 2 (SARS-CoV-2) enzyme linked immunosorbent assays (ELISA) and one pseudotype lentivirus-based neutralization test (ppNAT) in detecting the convalescent plasma antibody levles from COVID-19. 【Methods】 30 COVID-19 convalescent plasma samples were screened for antibodies against SARS-CoV-2 using three kinds of SARS-CoV-2 ELISA reagents and one ppNAT test in Shenzhen. The controls consisted of plasma samples from 32 healthy blood donors in February 2019. The diagnostic efficacy analysis of various SARS-CoV-2 ELISA reagents was performed using real-time fluorescent Polymerase Chain Reaction (RT-PCR). We also analyzed correlation between different immunological reagents and the age, gender, hospitalization, and severity of illness. 【Results】 The positive yielding rate of ppNAT and three kinds of IgG ELISA was higher than that of IgM ELISA. The positive yielding rates of three kinds of IgG ELISA were 100%(30/30), 93.33%(28/30), and 96.67%(29/30) respectively, while the yielding rates in control group were all 0. The positive yielding rate of three IgM ELISAs were 93.33%(28/30), 70%(21/30)and 46.67% (14/30). All the cases from negative control group were negative for IgG and IgM. Pearson correlation coefficient was calculated; there was a strong correlation between ELISA reagent 2 IgG and ELISA reagent 3 IgG (r=0.765, P<0.01). The correlation between ppNAT, ELISA reagent 3 IgG and the age of recovered patients was 0.422 and 0.385, respectively (P<0.05), while no significant correlation was found between the duration of hospitalization, severity of illness, gender and antibody signal/cutoff (S/CO) (P>0.05). 【Conclusion】 In the convalescent plasma with nucleic acid confirmed covid-19, the yielding rates of different IgM antibodies varied greatly. Antibody levels were influenced by age to some extent.
