Preparation of cryoprecipitate coagulation factor from liquid-state plasma by supercooling method
10.13303/j.cjbt.issn.1004-549x.2021.11.026
- VernacularTitle:利用过冷方法从液态血浆制备冷沉淀凝血因子的研究
- Author:
Wei KANG
1
;
Delong LI
1
;
Dong WANG
1
;
Manhua ZHOU
2
;
Juanjuan LI
2
Author Information
1. Dalian Blood Center, Dalian 116001, China
2. Dalian Medical University
- Publication Type:Journal Article
- Keywords:
supercooling;
cryoprecipitate coagulation factors;
fibrinogen;
coagulation factor Ⅷ
- From:
Chinese Journal of Blood Transfusion
2021;34(11):1261-1262
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To establish a novel preparation method of cryoprecipitate coagulation factor from overcooled liquid-state plasma. 【Methods】 The fresh liquid plasma was kept at -11℃ to -13℃ for a period of time. It can remain in the liquid state with some coagulation factors generated due to supercooling. Then cryoprecipitate can be obtained from the liquid plasma by siphon method. 【Results】 The average fibrinogen content yielded in cryoprecipitate, prepared from 50 samples of 16-hour-stored fresh liquid plasma, was (186.02±22.72) mg, with the average recovery rate of (37.51±7.42) %, and the average content of coagulation FⅧ was (104.66±22.88) IU, with the average recovery rate of (46.62±5.58) %. 【Conclusion】 The cryoprecipitate coagulation factors could be obtained not only from fresh frozen-thawed plasma, but also from overcooled liquid plasma which is simple and stable, also meets the requirements of relative standards.
