Analysis of HBV serological markers of donor blood samples implicated in TMA triplex + but discriminatory test-samples
10.13303/j.cjbt.issn.1004-549x.2021.11.016
- VernacularTitle:TMA联检反应性而鉴别非反应性献血者标本HBV血清学标志物分析
- Author:
Suping LI
1
;
Mingrui LI
1
;
Kai WANG
1
;
Tingting WANG
1
;
Qin LÜ
1
;
Dequn NI
1
;
Ting WANG
1
;
Xin XIE
1
;
Weifang CHENG
1
Author Information
1. Anhui Blood Center, Hefei 230031, China
- Publication Type:Journal Article
- Keywords:
blood donor;
hepatitis B virus;
nucleic acid testing;
serological markers
- From:
Chinese Journal of Blood Transfusion
2021;34(11):1228-1231
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the status of HBV infection and low viral load of HBV DNA in blood donor samples implicated in TMA triplex reactive but discriminatory test non-reactive samples. 【Methods】 A total of 51 996 samples were detected by Procleix Panther nucleic acid detection(NAT) system from January 2020 to March 2021, and 86 of them were TMA triplex reactive but discriminatory test non-reactive. HBV serological markers (HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc) were detected by electrochemiluminescence. Single-donation(ID) NAT was conducted in some TMA triplex + /discriminatory test-samples using Roche NAT. 【Results】 Out of 86 TMA triplex + /discriminatory test-samples, anti-HBc were positive in 89.53% (77/86), anti-HBe positive in 27.90% (24/86) and anti-HBs positive in 65.12% (56/86). 15 donors carried anti-HBs, anti-HBe and anti-HBc, 34 both anti-HBs and anti-HBc, 1 both anti-HBs/anti-HBe, 8 both anti-HBe/anti-HBc, 6 solo anti-HBs, and 20 solo anti-HBc. The positive rate of HBV serological markers was 97.67% (84/86). HBV DNA in 5 out of the 10 samples was qualitatively detected by Roche ID NAT, and one of them presented HBV DNA < 20 IU/mL. 【Conclusion】 Most TMA triplex + /discriminatory test-samples were occult hepatitis B infection.
