Evaluation of modified IAT with twoself-made low ionic-strength solutions
10.13303/j.cjbt.issn.1004-549x.2021.11.004
- VernacularTitle:自制增强剂在间接抗球蛋白试验中的临床应用
- Author:
Tianshu JIANG
1
;
Leiyu CHEN
1
;
Ye LIU
1
;
Yin LIU
1
;
Ye ZHOU
1
;
Xiaofeng TANG
1
Author Information
1. Department of Blood Transfusion, Changzheng Hospital, Naval Medical University, Shanghai 200003, China
- Publication Type:Journal Article
- Keywords:
low ionic strength solution;
polyethylene glycol;
bovine serum albumin;
indirect antiglobulin test
- From:
Chinese Journal of Blood Transfusion
2021;34(11):1190-1192
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the clinical applications of low ionic strength salt solution polyethylene glycol (LISS-PEG) and low ionic strength salt solution bovine serum albumin (LISS-BSA) in the indirect antiglobulin test (IAT). 【Methods】 The common standard red blood cell(RBC) IgG irregular antibodies (anti-D, anti-M, anti-N, anti-S, anti-s, anti-Jka, anti-Jkb, anti-Fya, anti-Fyb, anti-Dia, anti-K) were reacted with RBCs with corresponding antigens in IAT with LISS-PEG and LISS-BSA modification for 5-minute and 10-minute incubations, and then compared the results with conventional IAT. One hundred of blood samples from patients presenting irregular antibodies of erythrocyte IgG were selected to observe the effect of these two self-made modification. The agglutination intensity was recorded by AABB scoring method. 【Results】 No difference was noticed in IAT intensity reaction between LISS-PEG 5-minute and 15-minute incubation (P>0.05), nor between 5-minute/15-minute LISS-BSA incubation and conventional IAT (P>0.05). However, LISS-PEG modification demonstrated a significant superiority over the conventional technique just after incubation with 5 minutes(P<0.05). 【Conclusion】 Using the self-made LISS-PEG as the enhancement medium allows not only reduced incubation time (5 minutes) but also increased intensity of the reaction, which shortens the cross-matching time for emergency blood transfusion and is worthy of popularization.
