Research progress on thrombogenesis induced by human stem cells in vitro
10.13303/j.cjbt.issn.1004-549x.2021.12.035
- VernacularTitle:体外人干细胞分化血小板研究进展
- Author:
Weihua HUANG
1
;
Ruoru WANG
2
;
Haihui GU
1
;
Baohua QIAN
1
Author Information
1. Department of Transfusion Medicine, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, China
2. Department of Neurology, The First Affiliated Hospital of Naval Medical University, Shanghai 200433, China
- Publication Type:Journal Article
- Keywords:
thrombogenesis;
in vitro experiment;
megakaryocytes;
human stem cells;
thrombocytopenia
- From:
Chinese Journal of Blood Transfusion
2021;34(12):1403-1408
- CountryChina
- Language:Chinese
-
Abstract:
Platelet transfusion is the main clinical treatment of thrombocytopenia. However, due to the difficulty of platelet collection, high cost of use and limited number of blood donors, the development of platelet treatment is greatly limited. Therefore, the research on thrombogenesis in vitro has attracted more attention at home and abroad. Platelet production in vitro has the advantages of donor-independence, platelet antigen free and low risk of alloimmunity. At present, the efficiency of producing functional platelets in vitro is low, and there is still a big gap to achieve the ultimate goal of producing a large number of functional platelets in vitro. This paper reviews the research progress of megakaryocyte / platelet production in vitro, focuses on the in vitro production potential of megakaryocyte / platelet, and summarizes the current platelet culture systems in vitro based on human pluripotent stem cells, embryonic stem cells and adipose stem cells. The contradictions and difficulties of platelet production in vitro were also discussed to provide theoretical support for further research.
