HBV DNA load detected by two domestic fluorescent quantitative PCR reagents: a comparative analysis
10.13303/j.cjbt.issn.1004-549x.2021.12.025
- VernacularTitle:两种国产荧光定量PCR试剂检测HBV DNA载量的对比分析
- Author:
Ting LIU
1
;
Taotao JIA
1
;
Fei YANG
2
;
Huaiyong ZHANG
2
;
Jianming WU
2
;
Yanhui TONG
2
;
Yingjie QI
1
Author Information
1. The First Affiliated Hospital of Science and Technology of China, Anhui Provincial Hospital Infection Hospital, Hefei 230000, China
2. Geneway Biotechnology Co Ltd.
- Publication Type:Journal Article
- Keywords:
in vitro diagnostic reagent;
HBV DNA;
fluorescence quantification PCR
- From:
Chinese Journal of Blood Transfusion
2021;34(12):1374-1377
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To compare the quantitative detection results of two domestic quantitative real-time PCR reagents in HBV-DNA detection. 【Methods】 A total of 306 serum samples form hepatitis B patients were selected for quantitative parallel detection of high-sensitiveity HBV-DNA using domestic reagent A and B, and the difference and consistency of the results were analyzed. 【Results】 1) The yielding rate of reagent A and B was 86.92% and 84.64%, respectively. The regression linear equation was Y=0.984 9x+ 0.154 9, R2=0.945 7, the correlation coefficient r=0.972 5, indicating the results by the two reagents had good correlation. 2) When the concentration was in the range of(20~1 000) IU/mL, the yielding rates of reagent A and B were 39.9% and 37.6%, respectively. 【Conclusion】 Reagent A is more suitable for post-treatment monitoring in patients with OBI and HBeAg(-), but also can be used to detect HBV pathogens in patients before operations or blood transfusions.
