β-lactam antibiotics detection using microcolumn gel technology
10.13303/j.cjbt.issn.1004-549x.2021.12.005
- VernacularTitle:微柱凝胶技术用于检测β-内酰胺类药物抗体的研究
- Author:
Xiaona LI
1
;
Chengming MA
1
;
Jing CHEN
1
;
Junli ZHAO
2
;
Yiran ZHAO
2
;
Meng GUO
2
;
Hongjuan PAN
2
;
Juyou LIANG
2
;
Qiaoling XING
2
;
Jing GUO
2
;
Xutong LI
2
Author Information
1. Department of Blood Transfusion, the Third Hospital of Hebei Medical University, Shijiazhuang 050051, China
2. Langfang Blood Center
- Publication Type:Journal Article
- Keywords:
β-lactam antibiotics;
microcolumn gel method;
drug antibody;
drug-induced anemia;
hemolytic anemia
- From:
Chinese Journal of Blood Transfusion
2021;34(12):1296-1299
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To develop an assay to determine β-lactam antibiotics using microcolumn gels and to study the β-lactam antibiotics present in the blood of patients and their clinical significances. 【Methods】 446 patients with a history of taking β-lactam antibiotics from January 2019 to June 2019 were randomly selected from Trauma Emergency Center, Department of Arthrosis, Department of Spine and Department of Bone Oncology of our hospital, and 4 mL(per capita) venous blood was collected. Irregular antibody screening, anti-globulin detection and drug antibody determination were performed by microcolumn gel method. The data of gender, age, disease, blood transfusion history and medication were collected. The test results and clinical data were retrospective analyzed. 【Results】 The yielding rate of antibody was 0.45%(2/446) in patients with a history of taking β -lactam antibiotics. 16.38%(73/446) of the samples were positive in direct antiglobulin test, and 64.38%(47/73) of them did not agglutinate with RBCs treated with drugs. The yielding rate of specific antibodies against drug was 4.93%(22/446), and the titer ranged from 2 to 128(8). 1 case of auto-IgM antibody, 1 case of blood group related antibody and 2 cases of non-specific protein adsorption were detected. The yielding rate of drug antibody in patients with blood transfusion history reached to 12.10 %(22/124), so it was also high in patients with bone tumor. 【Conclusion】 Direct antiglobulin assay is helpful for the detection of β-lactam antibodies. The negative results of antibody screening cannot completely exclude the presence of drug antibodies. The yielding rate of drug antibody can be greatly improved by specific drug antibody detection, and it was higher in transfused patients relative to non-transfused one.
