Serological characteristics and genotype analysis of D epitope blood group of RhD variant individuals in Guangzhou
10.13303/j.cjbt.issn.1004-549x.2021.12.004
- VernacularTitle:广州地区RhD变异型个体D抗原表位血型血清学特征及基因型分析
- Author:
Boquan HUANG
1
,
2
;
Shuangshuang JIA
3
;
Jizhi WEN
3
;
Guangping LUO
3
;
Yanli JI
3
;
Huayou ZHOU
1
Author Information
1. The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China
2. Institute of Clinical Blood Transfusion, Guangzhou Blood Center
3. Institute of Clinical Blood Transfusion, Guangzhou Blood Center
- Publication Type:Journal Article
- Keywords:
Rh blood group;
RhD variants;
epitopes of RhD antigen;
serology;
molecular biology;
MLPA
- From:
Chinese Journal of Blood Transfusion
2021;34(12):1290-1295
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the characteristics of the D antigen epitope of individuals with RhD variants and the genetic molecular mechanism of gene mutations in Guangzhou. 【Methods】 A total of 59 samples of RhD variants were collected from blood donors and hospitals in Guangzhou from January to August 2019. Serological characteristics of D epitopes were further analyzed using two kinds of monoclonal anti-D reagents and D epitope detection kits, and RHCE phenotypic typing was performed. QuickGene DNA extraction kit was used to extract the genomic DNA of the samples, and PCR-RFLP method was used to analyze the RHD gene zygote type. The RHD gene sequence was detected by multiple ligation-dependent probe amplification(MLPA) genotyping, and the RHD exon(1~10) Sanger sequencing was performed on the samples still in doubt after the above detection. DNAStar/SeqMan analysis software was used for comprehensive analysis. 【Results】 In this group of individuals with RhD variants in Guangzhou, 27.12%(16/59) were detected from blood donors [accounting for 0.007%(16/232 793) of blood donors in Guangzhou during the same period], and difficult samples of patients sent by hospitals for determination accounted for 72.88%(43/59). RHD genotype detection: 40.68%(24/59) were RHD*weak partial 15, 25.42%(15/59) were RHD* DⅥ.3 and 33.90%(20/59) were rare RHD variants [76.92%(10/13) were RhD variants with 2 different alleles]. Serological D-screen revealed a relatively fixed pattern of RHD*DⅥ.3 in anti-D antibody(clone: P3*212 23B10), while the others was negative. The phenotypic distribution of RhD variant CE was Ccee 38.98%(23/59), ccEe 35.59%(21/59), CcEe 25.42%(15/59). 【Conclusion】 Weak partial D15 and DⅥ.3 were the most common RhD variants in Guangzhou Han population, and DⅥ can be preliminarily identified by serological methods such as D-Screen anti-D reagent, while the remaining RhD variants can only be identified by molecular biological methods, and >95% of the RhD variants were C+ or E+ phenotypes.
