Serological and molecular biological analysis of RhD--: a case report
10.13303/j.cjbt.issn.1004-549x.2023.12.020
- VernacularTitle:一例RhD－－缺失型患者的血清学及分子生物学分析
- Author:
Bo SHUI
1
;
Zhibing HOU
1
;
Li YAO
1
;
Wei YAN
2
;
Jiwu HE
2
Author Information
1. Xiangyang Central Blood Bank, Xiangyang 441021, China
2. Department of Blood Transfusion, Xiangyang No.1 People′s Hospital, Hubei University of Medicine
- Publication Type:Journal Article
- Keywords:
Rh phenotype;
serological test;
gene sequencing;
RHCE;
RHAG
- From:
Chinese Journal of Blood Transfusion
2023;36(12):1162-1164
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To study the blood group serology and molecular biology of patients with RhD--, so as to guide clinical blood use. 【Methods】 The EDTA-K2 anticoagulant blood of the patient was detected for Rh antigens and antibodies. Meanwhile, DNA was extracted, and the 1-10 exon of RHCE and RHAG gene was sequenced by Sanger sequencing. 【Results】 The serological test showed O type RhD--, and all spectral cells were positive. RHCE gene sequencing showed RHCE*02/RHCE*02, RHAG gene sequencing showed mutations at site 808 G > A and site 861 G > A of exon 6. 【Conclusion】 When patients were with RhD--, and related immune conditions such as pregnancy and/or transfusion history were present, autologous blood transfusion or plasma exchange could be an option for emergency blood use.
