Diversity of IgG autoantibodies of 4 Chinese intravenous immunoglobulins
10.13303/j.cjbt.issn.1004-549x.2023.12.005
- VernacularTitle:4种静注人免疫球蛋白制品的自身IgG抗体多样性研究
- Author:
Xiaochen YAN
1
;
Li MA
1
;
Zongkui WANG
1
;
Changqing LI
1
;
Tingting LEI
1
;
Xi DU
1
;
Shengliang YE
1
Author Information
1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China
- Publication Type:Journal Article
- Keywords:
intravenous immunoglobulin(IVIG);
IgG autoantibody;
antibody repertoire;
diversity of antibodies
- From:
Chinese Journal of Blood Transfusion
2023;36(12):1102-1106
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To develop methods to display the IgG autoantibody repertoire of intravenous immunoglobulin (IVIG) products, analyze the different types of antibodies and study the diversity of IgG autoantibody in 4 IVIG preparations from different Chinese manufacturers. 【Methods】 Two-dimensional gel electrophoresis and immunoblotting with human umbilical vein endothelial cell (HUVEC) proteins were used to demonstrate the IgG autoantibody repertoire and the human protein microarray with bioinformatics analysis was employed to profile the immune reactive autoantigens of the 4 IVIG preparations. 【Results】 The methods to showcase the autoantibody repertoire and study the antibody diversity of IVIG were successfully established. High-quality repertoires of IVIG autoantibodies and biological information about self-proteins that can be recognized were obtained. There was a significant difference in the recognition of the quantity and variety of the self-antigens by different IVIG products. The number of antibodies against HUVEC proteins in four products ranged from 241-386. The number of proteins recognized on the human protein chip ranged from 292-435, with 172 human self-proteins recognized by all four products. 【Conclusion】 Demonstration of antibody repertoire and protein chip technology can be used to analyze IVIG products′ IgG autoantibody repertoire. All four preparations tested in this study exhibited a broad spectrum of antibodies against HUVEC proteins and human proteome microarray, each product had its unique antibody repertoire characteristics.
