False positive reaction of COVID-19 antibody ELISA screening: internal logic of various methods
10.13303/j.cjbt.issn.1004-549x.2023.12.001
- VernacularTitle:新冠病毒抗体ELISA筛查假阳性反应：不同方法检测结果的内在逻辑
- Author:
Lilin WANG
1
;
Ran LI
1
;
Tong LI
1
;
Aijia ZHANG
1
;
Heng LIU
1
;
Xiaoxuan XU
1
;
Jinfeng ZENG
1
;
Linfeng WU
1
Author Information
1. Shenzhen Blood Center, Shenzhen 518000, China
- Publication Type:Journal Article
- Keywords:
severe acute respiratory syndrome coronavirus 2(SARS-CoV-2);
antibody reactivity samples;
pseudovirus neutralizing antibody test;
Western blot(WB);
dynamic monitoring
- From:
Chinese Journal of Blood Transfusion
2023;36(12):1085-1089
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the results of different methods for reactive samples screened by the enzyme linked immunosorbent assay (ELISA) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in blood donors. 【Methods】 From March to April 2020, a total of 8 632 blood samples in Shenzhen were screened for SARS-CoV-2 total antibodies (TAb, including IgG, IgM, IgA) in plasma using ELISA(PC group), the antibody reactivity samples and their follow up plasma samples (FC group), and samples of disease control group(DC group) from January to April 2020 were detected using the following methods: 1) ELISA method for detecting IgG, IgM, and (or without detection) TAb; 2) pseudovirus neutralizing antibody test(pVNT); 3) western blot (WB) of SARS-CoV-2 antibody. The negative control group(NC group) from February to April 2020 performed ELISA and WB testing. 【Results】 Among the 34 total antibody positive samples, 2 were positive for pVNT test, and the total antibody, IgG and WB in the initial screening and tracking testing were positive. Thereafter, it was determined to be confirmed positive. The other 2 cases were positive for pVNT test, while the samples with positive WB results were in the follow-up stage. The TAb, IgG, and pVNT results did not conform to the dynamic evolution of antibodies, and cannot be determined as confirmed positive. 【Conclusion】 The infection status of antibody reactivity samples screened by SARS-CoV-2 ELISA can be judged by the logic of pVNT, WB and the dynamic change of antibody.
