HIV-, HBV- and HCV- NAT yield in individual donations: a multi-center analysis in Henan province from 2017 to 2019

Yan Zhang; Jianhua Fang; Wenchao GE; Yifang Wang; Qi Jiang; Shanhua Chen; Dan Chen; Jia WU; Nan Chen; Yu Meng; Huiyun Han; Dengfeng Wang; Xiaozhen Zhang; Yue Zhang; Fengyong MA; Bin Jia; Weijie LI; Sili Sun; Fei Zhao; Yongqing Zhou; Suneng Zhang

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HIV-, HBV- and HCV- NAT yield in individual donations: a multi-center analysis in Henan province from 2017 to 2019

VernacularTitle:河南省18家血站2017～2019年HIV/HBV/HCV核酸单独阳性结果趋势性分析
Author: Yan ZHANG ¹ ; Jianhua FANG ¹ ; Wenchao GE ¹ ; Yifang WANG ¹ ; Qi JIANG ² ; Shanhua CHEN ³ ; Dan CHEN ⁴ ; Jia WU ⁵ ; Nan CHEN ⁶ ; Yu MENG ⁷ ; Huiyun HAN ⁸ ; Dengfeng WANG ⁹ ; Xiaozhen ZHANG ¹⁰ ; Yue ZHANG ¹¹ ; Fengyong MA ¹² ; Bin JIA ¹³ ; Weijie LI ¹⁴ ; Sili SUN ¹⁵ ; Fei ZHAO ¹⁶ ; Yongqing ZHOU ¹⁷ ; Suneng ZHANG ¹⁸
Author Information

1. Henan Red Cross Blood Center, Zhengzhou 450052, China
2. Kaifeng Central Blood Station
3. Luoyang Central Blood Station
4. Pingdingshan Central Blood Station
5. Anyang Central Blood Station
6. Hebi Central Blood Stationr
7. Xinxiang Central Blood Station
8. Jiaozuo Central Blood Station
9. Puyang Central Blood Station
10. Xuchang Central Blood Station
11. Luohe Central Blood Station
12. Sanmenxia Central Blood Station
13. Shangqiu Central Blood Station
14. Zhoukou Central Blood Station
15. Zhumadian Central Blood Station
16. Nanyang Central Blood Station
17. Xinyang Central Blood Station
18. Jiyuan Central Blood Station
Publication Type:Journal Article
Keywords: nucleic acid testing(NAT); NAT reactive to isolated infectious virus; Henan province; blood donors; , quality control
From: Chinese Journal of Blood Transfusion 2021;34(1):68-72
CountryChina
Language:Chinese
Abstract: 【Objective】 To analyze the HIV-, HCV- and HBV- NAT yield rate in different areas of Henan province, so as to provide the basis for disease prevention and control as well as the establishment of a unified quality control standard for nucleic acid testing(NAT) in the Henan province. 【Methods】 The number and prevalence of NAT yielding samples with isolated infectious virus, namely HIV, HCV and HBV, in 18 blood stations in Henan province from 2017~2019, as well as the trends were analyzed. The NAT quality of each laboratory and each testing system was analyzed according to the ratio of reactive individual donation(ID) results to reactive minipools(MP). 【Results】 The HBV, HCV and HIV ID-NAT yield numbers in 3 501 251 blood donations were HBV 2 606(74/100 000), HCV 21 (0.63/100 000), and HIV 34(1.00/100 000). The HBV ID-NAT yield rate showed an upward trend in the whole province from 2017 to 2019, while the prevalence of HIV and HCV ID-NAT yield didn′t differ significantly during three years. 5 kinds of NAT detection systems were applied in 18 blood centers. among which Ⅰ, Ⅱ, Ⅳ and Ⅴ were triplex detection systems. 2661 ID-reactive samples were implicated in 5 595 MP-reactive samples, with a resolution rate of 47.56%. The resolution rate of triplex NAT system Ⅰ, Ⅱ, Ⅳ and Ⅳ was 39.63%~47.95%, 40.43%~54.36%, 51.61% and 70.00%~45.45%, respectively. An upward trend in triplex NAT resolution rate was observed in 8 laboratories, i. e.B, D, E, F, I, K, L and Q, and an descending trend in A and C. The NAT system Ⅲ, a ID-NAT system, was used only by laboratory C, presenting a NAT-yield rate of 0.19% (282/145 474) and resolution rate of 46.45% (131/282). 【Conclusion】 The majority of NAT-yield of one infectious virus in Henan province is HBV, presenting annual increasing trend. The quality management of NAT laboratories should be strengthened as the divergence was seen in the performance of different NAT laboratories.