Correlation between adhesion molecules on the surface of red blood cell membrane and red blood cell lifespan
10.13303/j.cjbt.issn.1004-549x.2021.01.006
- VernacularTitle:红细胞膜表面黏附分子与红细胞寿命的相关性研究
- Author:
Peng FANG
1
,
2
;
Ling LI
3
,
4
;
Rui HE
3
,
4
;
Jiajia QIAO
3
,
4
;
Li GONG
1
,
4
;
Zhong LIU
1
,
2
,
4
Author Information
1. Anhui Medical University, Hefei 230032, China
2. Key Laboratory of Adverse Reactions, CAMS
3. Key Laboratory of Adverse Reactions, CAMS
4. Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College
- Publication Type:Journal Article
- Keywords:
red blood cell lifespan;
cell adhesion molecules;
biomarkers;
red blood cell membrane surface;
Percoll density gradient centrifugation
- From:
Chinese Journal of Blood Transfusion
2021;34(1):19-22
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the correlation between red blood cell lifespan and adhesion molecules on the surface of red blood cell membrane, in order to establish a method to detect the duration of red blood cell storage. 【Methods】 10 samples(10 mL each) of fresh red blood cell, collectedf rom 10 healthy voluntary blood donors, were divided into 5 age groups (layers) by Percoll density gradient centrifugation. The expression of CD47, CD44 and CD147 on the surface of red blood cell membrane in each layer was detected using flow cytometry. The variance of protein expression in each layer of red blood cells was analyzed by SPSS statistical software. 【Results】 The expression levels (%) of 3 adhesion molecules on the surface of red blood cell membranes from young to old were CD47: 14.44±2.61, 9.30±1.75, 7.84±1.49, 6.54±1.32 and 5.53±1.12 (P<0.01); CD44: 25.01±1.94, 19.22±1.52, 17.10±1.28, 15.18±1.11 and 13.56±1.08 (P<0.01); CD147: 33.46±1.99, 28.31±2.95, 23.83±1.59, 20.40±1.56 and 18.03±1.65 (P<0.01). 【Conclusion】 The expression levels of CD47, CD44 and CD147 on the surface of red blood cell membranes have showed a downward trend as the storage extended. These three protein adhesion molecules have showed a correlation with red blood cells lifespan, and could be used as detection markers of cell age.
