Simultaneous determination of six aniline compounds in workplace air by high performance liquid chromatography
10.20001/j.issn.2095-2619.20230814
- VernacularTitle:高效液相色谱法同时测定工作场所空气中6种苯胺类化合物
- Author:
Ruibo MENG
1
;
Jingjing QIU
;
Weifeng RONG
;
Weimin XIE
;
Jing YUAN
;
Jiaheng HE
;
Jiawen HU
;
Ziqun ZHANG
;
Zuokan LIN
;
Fan WU
;
Banghua WU
Author Information
1. Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, Guangdong 510300, China
- Publication Type:Journal Article
- Keywords:
Workplace;
Air;
Aniline;
Compounds;
Co-existing;
Silica gel;
Filter membrane;
High performance liquid chromatography
- From:
China Occupational Medicine
2023;50(4):441-446
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatography (HPLC) method for simultaneous determination of six aniline compounds (ADs) in workplace air. Methods GDH-1 air sampling tube was used to collect six co-existing ADs such as aniline, o-toluidine, N-methylaniline, m-methylaniline, p-methylaniline and N,N-dimethylaniline in the vapor and aerosol of workplace air. The samples were desorbed and eluted using a methanol solution containing 1.00% ammonia water, followed by separation on a C18 chromatographic column and detection using a diode array detector. Results The quantification range of the method was 0.19 -253.50 mg/L, with the correlation coefficient of 0.999 9 for all six ADs. The minimum detection range was 0.02-0.06 mg/m3, and the minimum quantitation range was 0.04-0.19 mg/m3 [both calculated for a 15.0 L sample with a desorption (elution) solution volume of 3.00 mL]. The average desorption and elution efficiencies were 92.15%-104.41% (silica gel) and 94.29%-104.29% (filter membrane). The intra-assay relative standard deviation (RSD) ranged from 0.90%-9.72% (silica gel) and 0.57%-6.96% (filter membrane). The inter-assay RSD ranged from 2.03%-9.78% (silica gel) and 2.50%-8.62% (filter membrane). The samples were stable at room temperature for seven days. Conclusion This method can be used for the simultaneous determination of six ADs in workplace air.
