Identification and Expression Pattern of Salvia miltiorrhiza Terpenoid Synthase Gene Family
10.13422/j.cnki.syfjx.20231612
- VernacularTitle:丹参萜类合酶基因家族的鉴定和表达模式分析
- Author:
Ying GAO
1
;
Jinzhu JIANG
1
;
Yingying GAO
2
;
Hongtao YANG
3
;
Xianju LIU
1
;
An LIU
1
Author Information
1. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
2. Institute of Botany， Chinese Academy of Sciences， Beijing 100093， China
3. China Chemical Safety Association， Beijing 100013， China
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhiza;
terpenoid synthase （TPS）;
bioinformatics;
function analysis;
gene family
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(3):142-151
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveBioinformatics methods were used to systematically identify the Salvia miltiorrhiza terpenoid synthase （SmTPS） gene family members and predict their functions from the perspective of the genome. MethodThe genome and transcriptome data of S. miltiorrhiza， Arabidopsis thaliana， and tomato were obtained from the national genomics data center （NGDC）， national center for biotechnology information （NCBI）， the Arabidopsis information resource （TAIR）， and tomato functional genomics database （TFGD）， and the whole genome identification and bioinformatics analysis of the SmTPS gene family member were carried out with the help of Perl language programming， Tbtools， and other bioinformatics tools. ResultA total of 52 TPS gene family members were identified， and they were distributed on eight chromosomes of S. miltiorrhiza. Their coding amino acid number was 207-822 aa. The isoelectric points were 4.76-9.16. The molecular mass was 24.11-94.81 kDa， and all members are hydrophilic proteins. Gene structure analysis showed that there were significant differences in the number of introns among different subfamilies. The number of introns in 72.6% of TPS-a， b， and g subfamilies was 6， and that in 88.9% of TPS-c and e/f subfamilies was more than 10. Protein motifs were conserved among TPS subfamilies. The analysis of promoter cis-acting elements showed that all promoters of the SmTPSs contained a large number of light-responsive elements， and most of them had hormone-responsive elements. Gene expression analysis showed that SmTPS gene family members exhibited tissue-specific expression， and 24 of them responded to exogenous methyl jasmonate. ConclusionBased on the published S. miltiorrhiza genome， 52 SmTPS gene family members were identified， and their functions were predicted based on the phylogenetic analysis and expression patterns. This paper provides reference information for the further biosynthesis pathway and regulatory mechanism analysis of terpenoids in S. miltiorrhiza.
