Analysis on Components Absorbed into Blood and Cerebrospinal Fluid of Schisandrae Chinensis Fructus Based on Sequential Metabolism
10.13422/j.cnki.syfjx.20230967
- VernacularTitle:基于序贯代谢的五味子入血及脑脊液成分分析
- Author:
Shuang YU
1
;
Yanli PAN
2
;
Huining LIU
1
;
Xueyan LI
1
;
Xinyu WANG
1
;
Dongying QI
1
;
Fulu PAN
1
;
Qianqian WANG
1
;
Xiaoyu CHAI
1
;
Guopeng WANG
3
;
Tao MA
1
;
Yang LIU
1
Author Information
1. School of Chinese Materia Medica，Beijing University of Chinese Medicine，Beijing 102488，China
2. Institute of Information on Traditional Chinese Medicine，China Academy of Chinese Medical Sciences，Beijing 100700，China
3. Zhongcai Health（Beijing） Biological Technology Development Co. Ltd.，Beijing 101500，China
- Publication Type:Journal Article
- Keywords:
Schisandrae Chinensis Fructus;
sequential metabolism;
in vivo metabolism;
cerebrospinal fluid;
central nervous system;
ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry（UPLC Q-Exactive Orbitrap MS）;
plasma
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(3):114-123
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify the prototypical components and metabolites absorbed into blood and cerebrospinal fluid of Schisandrae Chinensis Fructus（SCF） based on sequential metabolism combined with liquid chromatography-mass spectrometry. MethodBlood and cerebrospinal fluid samples of integrated metabolism， intestinal metabolism and hepatic metabolism were collected from male SD rats after gavage and in situ intestinal perfusion administration， and ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry（UPLC Q-Exactive Orbitrap MS） was used to analyze and compare the differences in the spectra of SCF extract， blank plasma， administered plasma， blank cerebrospinal fluid and administered cerebrospinal fluid with ACQUITY UPLC BEH Shield RP18 column（2.1 mm×100 mm， 1.7 µm）， the mobile phase was acetonitrile（A）-0.1% formic acid aqueous solution（B） for gradient elution（0-7 min， 95%B； 7-12 min， 95%-35%B； 12-17 min， 35%-15%B； 17-20 min， 15%-12%B； 20-22 min， 12%-5%B； 22-23 min， 5%B； 23-25 min， 5%-95%B； 25-28 min， 95%B）. And heated electrospray ionization（HESI） was used with positive and negative ion modes， the scanning range was m/z 100-1 500. The prototypical constituents and their metabolites absorbed into blood and cerebrospinal fluid of SCF were identified according to the retention time， characteristic fragments， molecular formulae and the information of reference substances. ResultA total of 42 chemical components were identified in the extract of SCF， including lignans， flavonoids， amino acids， tannins， and others， of which lignans were the main ones. A total of 27 prototypical components and 14 metabolites were identified in plasma samples from different sites. A total of 15 prototypical components and 9 metabolites were identified in cerebrospinal fluid. The main metabolic reactions involved in the formation of metabolites were mainly demethylation， methylation， demethoxylation and hydroxylation. ConclusionThrough the systematic identification of the prototypical components and metabolites of SCF in rats， it provides data support for further better exploring the material basis of SCF in the treatment of central nervous system diseases.
