In vitro release of paclitaxel derivative liposome by paddle membrane binding assay
10.11665/j.issn.1000-5048.2023041803
- VernacularTitle:类紫杉醇脂质体体外释放的桨膜结合分析法研究
- Author:
Yuting ZHENG
1
;
Tao HONG
;
Kehui XU
;
Minghao WEN
;
Jixue YANG
;
Mengying WU
;
Taijun HANG
;
Min SONG
Author Information
1. 中国药科大学药物分析系
- Publication Type:Journal Article
- Keywords:
in vitro release of liposome;
dialysis method;
paclitaxel derivative liposome;
paddle membrane binding assay
- From:
Journal of China Pharmaceutical University
2023;54(6):743-748
- CountryChina
- Language:Chinese
-
Abstract:
The in vitro release is an important index to evaluate the quality of liposome formulation.Currently, there is no evaluation method for the in vitro release of liposome in pharmacopoeia of various countries, which leads to the lack of unified standard and safety guarantee for the quality evaluation of liposome formulation.Taking the self-made paclitaxel derivative liposomes as an example, the paddle membrane binding method established by optimizing external release conditions was used to simulate the complete release of paclitaxel derivative drugs in 12 hours under physiological conditions.The results showed that using 0.5% SDS-HEPES as the release medium and a dialysis bag with a molecular weight cutoff of 1 000 kD to release the liposome solution met the requirements and had discrimination ability, providing a reference for the development of drug-loaded liposomes release methods in vitro.
