An emerging analytical tool for highly sensitive quantitative analysis based on liquid chromatography-multiple-reaction monitoring cubed (MRM3)
10.11665/j.issn.1000-5048.2023062801
- VernacularTitle:基于液相色谱-多级多反应监测模式（LC-MRM3）的高灵敏定量分析新策略
- Author:
Ting LI
1
;
Ke ZHANG
;
Jun LI
;
Shengpeng WANG
;
Yuelin SONG
Author Information
1. 北京中医药大学北京中医药研究院
- Publication Type:Journal Article
- Keywords:
MRM3;
quantitative analysis;
complex matrices;
sensitivity;
LC-MS/MS
- From:
Journal of China Pharmaceutical University
2023;54(6):718-728
- CountryChina
- Language:Chinese
-
Abstract:
Liquid chromatography-multiple-reaction monitoring (LC-MRM) has been widely recognized as the golden standard for multiple components-targeted quantitative analysis of complicated matrices,with extensive applications for analysis in such fields as chemical drugs, traditional Chinese medicines and foods.Unfortunately, when facing the task of quantitatively analyzing trace chemical components in complex matrices, MRM suffers dramatically from the background noise or matrix interference, leading to undesirable sensitivity and selectivity in terms of the lower limits of quantification (LOQ) and detection (LOD).In recent years, MRM cubed (MRM3), also known as MS3 scan, has received much attention because of its unique ability to significantly improve detection selectivity and sensitivity attributing to the successive ion filtering function, enabling LC-MRM3 as an emerging analytical tool.In this review,our attention is devoted to: 1) the illustration of the principle for MRM3; 2) parameter settings; and 3) the application progress of LC-MRM3 in such fields as the pursuit of biomarkers, pharmaceutical analysis, forensic analysis, toxicological analysis, food chemistry, and environmental analysis, aiming to provide a promising analytical tool of LC-MRM3 advantageous in the quantification analysis of trace chemical components in complex matrices.
