Recent progress of protein glycosylation characterization utilizing native conformer-resolved mass spectrometry
10.11665/j.issn.1000-5048.2023060901
- VernacularTitle:蛋白质糖基化修饰的非变性构象分辨质谱研究进展
- Author:
Yifei JIA
1
;
Yamei WANG
;
Gongyu LI
Author Information
1. 南开大学化学学院
- Publication Type:Journal Article
- Keywords:
protein glycosylation;
native mass spectrometry;
conformer-resolving mass spectrometry;
ion mobility-mass spectrometry;
collision-induced unfolding
- From:
Journal of China Pharmaceutical University
2023;54(6):674-681
- CountryChina
- Language:Chinese
-
Abstract:
Glycosylation of proteins, one of the most prevalent and complex post-translational modifications occurring in nature, plays a crucial role in regulating protein net charge, conformation, binding properties and, ultimately, biological function.Traditional structural techniques are not amenable for glycoproteins due to the inherent heterogeneity of oligosaccharides.With the advances in analytical technique, mass spectrometry displays an increasingly crucial role in elucidating the structure of glycoproteins.Mass spectrometry-based proteomic technique can dissect the chemical composition and site information of low-abundance glycosylation at the peptide level.Instead, native mass spectrometry (nMS) can analyze intact glycoproteins while maintaining the information for glycan heterogeneity, and the insights into the regulatory effects of glycosylation on protein higher order structures and interactions with other proteins or ligands.As a representative structural mass spectrometry tool, ion mobility-based nMS strategy is powered by its conformer-resolving capability and by the feasibility of conformer manipulation through collision-induced unfolding.Consequently, native IM-MS analysis can provide rich information of dynamic protein conformations, allowing for the rapid identification and differentiation of protein isoforms in an unprecedented manner.In this review, we briefly introduced two emerging native IM-MS analytical modes, dynamic conformer-resolving mode and glycoform-resolving mode.Besides, we also discussed the recent progress of conformational and topological characterization of intact glycoproteins with three typical model systems based on two above-mentioned emerging modes of native IM-MS.
