Inhibition of CoCl2-induced glycolysis in HUVEC cells by heparin-derived oligosaccharides and its mechanism
10.11665/j.issn.1000-5048.2023042501
- VernacularTitle:肝素寡糖抑制CoCl2诱导的HUVEC细胞糖酵解及其机制
- Author:
Yuning QIN
1
;
Yuhao YAO
;
Joshua RUBAYIZA
;
HE HE
Author Information
1. 中国药科大学生命科学与技术学院
- Publication Type:Journal Article
- Keywords:
heparin-derived oligosaccharides;
glycolysis;
human umbilical vein endothelial cells;
hypoxia-inducible factor
- From:
Journal of China Pharmaceutical University
2023;54(5):626-633
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, cobalt chloride was used to stimulate human umbilical vein endothelial cells (HUVEC) to establish a model of abnormal hypoxic injury, to investigate the effect of heparin-derived oligosaccharides (HDO) on glycolysis in HUVEC cells and its molecular mechanism.The experiment was divided into the control group (FBS-free DMEM medium), the model group (FBS-free DMEM medium +50 μmol/L CoCl2), and the HDO group (modeling+0.01, 0.1, 1 μmol/L HDO).Firstly, a biochemical kit was used to detect the effects of HDO on glucose uptake and lactic acid accumulation in HUVEC cells, then Western blot and qPCR were used to detect the effects of HIF-1α, GLUT-1 and LDHA gene transcription and protein expression, and finally, PI3K/Akt signaling pathway was detected.The results showed that HDO inhibited glucose uptake and lactate production, down-regulated the expression of HIF-1α, GLUT-1, and LDHA, and affected the activation of the PI3K/Akt signaling pathway.HDO could regulate the glycolysis level of HUVEC cells by inhibiting the activation of the PI3K/Akt/HIF-1α signaling axis.
