Method establishment of component identification and content determination of 7 components in Mongolian medicine Ixeris chinensis
- VernacularTitle:蒙药材山苦荬的成分鉴别及7种成分含量测定方法建立
- Author:
Lijun SUN
1
;
Jun LI
1
,
2
;
Qiutong WANG
3
;
Huimin XIA
1
;
Yuewu WANG
1
,
2
;
Qian ZHANG
1
,
2
;
Huiwen ZHANG
1
;
Huanyun WANG
1
Author Information
1. College of Pharmacy，Inner Mongolia Medical University，Hohhot 010110，China
2. Inner Mongolia Autonomous Region Engineering Research Center of New Pharmaceutical Screening，Hohhot 010110，China
3. Drug Clinical Trial Institution，the Affiliated Hospital of Inner Mongolia Medical University，Hohhot 010110，China
- Publication Type:Journal Article
- Keywords:
Ixeris chinensis;
HPLC-Q-Exactive-MS;
HPLC-
- From:
China Pharmacy
2023;34(24):3006-3011
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the methods to identify the chemical components of Ixeris chinensis， and determine the contents of 7 components （chlorogenic acid， luteolin， quercetin， rutin， protocatechuic acid， isochlorogenic acid A， luteoloside）. METHODS HPLC-Q-Exactive-MS was used to identify the chemical components of I. chinensis. The contents of 7 components in I. chinensis， including chlorogenic acid， were determined by HPLC-MS/MS. RESULTS A total of 45 components were identified in I. chinensis， including 20 organic acids， 13 flavonoids， 4 fatty acids， 4 amino acids， 3 nucleosides， and 1 coumarin. The linear range of chlorogenic acid， luteolin， quercetin， rutin， protocatechuic acid， isochlorogenic acid A and luteoloside were 503.00- 25 150.00， 42.00-2 100.00， 5.05-252.50， 20.05-1 002.50， 25.10-1 255.00， 750.00-37 500.00， 196.00-9 800.00 ng/mL （r≥0.999 2）， respectively. RSDs of precision， stability and reproducibility tests were all less than 3.00% （n＝6）， and average recovery ranged from 96.72% to 105.84% （all RSD＜4.00%， n＝6）. The contents of 7 components in 3 batches of I. chinensis were 1 145.77- 3 261.25， 23.75-97.90， 0.92-2.12， 1.06-23.18， 9.35-21.85， 833.25-1 045.58， 199.56-1 869.78 μg/g， respectively. CONCLUSIONS The established methods for identification and content determination are rapid and simple， and can be used for the identification of chemical components and the content determination of 7 components in I. chinensis.
