Effect of fenofibrate on the expression of miR-26a-5p/PTEN in retinal neurons of diabetic mice
10.3980/j.issn.1672-5123.2024.1.05
- VernacularTitle:非诺贝特对糖尿病小鼠视网膜神经组织中miR-26a-5p/PTEN表达的影响
- Author:
Derong TANG
1
,
2
;
Yuwen YANG
1
,
2
;
Rui SHI
1
,
2
;
Dandan LIU
1
,
2
;
Rong LIN
1
,
2
Author Information
1. Medical College of Ankang Vocational and Technical College, Ankang 725000, Shaanxi Province, China
2. Department of Ophthalmology, Ankang Central Hospital, Ankang 725000, Shaanxi Province, China
- Publication Type:Journal Article
- Keywords:
diabetic retinal neurodegeneration;
miR-26a-5p;
phosphatase and tensin homologue(PTEN);
inflammation;
fenofibrate
- From:
International Eye Science
2024;24(1):24-29
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the protective effect of fenofibrate on diabetic retinal neurodegeneration and observe its effect on miR-26a-5p and its target gene PTEN in the retinal of diabetic mice.METHODS: Diabetic mice models were established and they were gavaged by fenofibrate. H& E staining and transmission electron microscopy were used to observe the impairments of retinal neurons. Real-time PCR was used to examine the expression of miR-26a-5p, and Western blotting was employed to measure the expression of phosphatase and tensin homologue(PTEN)in the retina of diabetic mice. The expression level of nuclear factor-κB(NF-κB), interleukin-1β(IL-1β)and the morphology of neural tissues were observed.RESULTS: When compared with the diabetic mice, fenofibrate significantly attenuated the damage to retinal ganglion cells and the atrophy of retinal nerve fiber layer. While the level of miR-26a-5p was increased and the levels of PTEN and inflammatory mediators were significantly decreased in the retina of fenofibrate treated diabetic mice, with significant statistical significance(P<0.05).CONCLUSIONS: Fenofibrate protects against diabetic retinal neurodegeneration by upregulating miR-26a-5p and inhibiting PTEN, attenuating the inflammatory response and alleviating retinal cell injury.
