Effect of low concentration of sodium fluoride on osteogenic/odontogenic differentiation of human dental pulp cells
10.12016/j.issn.2096-1456.2024.01.004
- Author:
LI Lifen
1
,
2
,
3
,
4
,
5
;
HAN Junli
1
,
2
,
3
,
4
,
5
;
JIANG Long
1
,
2
,
3
,
4
,
5
Author Information
1. Department of General Dentistry, Shanghai Ninth People'
2. s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine＆National Center for Stomatology &
3. National Clinical Research Center for Oral Disease &
4. Shanghai Key Laboratory of Stomatology &
5. Shanghai Research Insititute of Stomatology
- Publication Type:Journal Article
- Keywords:
human dental pulp cell / sodium fluoride / proliferation / osteogenic/odontogenic differentiation / endoplasmic reticulum stress / splicing xbox binding protein 1 / activating transcription factor 4 / glucose-regulated protein 78 / RNA-activated protein kinase-like ER-resident kinase / eukaryotic initiation factor-2α
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2024;32(1):22-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of low concentrations of sodium fluoride on the osteogenic/odontogenic differentiation of human dental pulp cells (hDPCs) in vitro.
Methods:This study was reviewed and approved by the Ethics Committee. hDPCs were cultured using a modified tissue explant technique in vitro. The effects of different concentrations of sodium fluoride on the proliferation of hDPCs were measured by methylthiazol tetrazolium (MTT) assay. Appropriate concentrations were added to the osteogenic/odontogenic differentiation induction medium, and the cells were induced in vitro. Alizarin red S staining was used to detect the osteoblastic/odontogenic differentiation ability of the cells, and the mRNA expression of the key differentiation factors was detected by RT-qPCR. Moreover, the expression of key molecules of endoplasmic reticulum stress (ERS) was detected by RT-qPCR and Western blot. The data were analyzed with the SPSS 18.0 software package.
Results:Low concentration of NaF (0.1 mmol/L) could stimulate cell proliferation in vitro, while a high concentration (5-10 mmol/L) could inhibit cell proliferation (P<0.05). According to the literature and the experimental data, 0.1 mmol/L NaF was selected as the following experimental concentration. The levels of alizarin red S staining were increased after NaF induction of mixed osteogenic/odontogenic differentiation in vitro. The mRNA expression levels of key molecules for osteogenic/odontogenic differentiation, dentin sialophosphoprotein (DSPP), bone sialoprotein (BSP) and osteocalcin (OCN), were increased (P<0.05). The mRNA levels of ERS markers (splicing x-box binding protein-1 (sXBP1), glucose-regulated protein 78 (GRP78) and activating transcription Factor 4 (ATF4) were increased in NaF-treated cells. The protein expression levels of key ER stress molecules (phosphorylated RNA-activated protein kinase-like ER-resident kinase (p-PERK), phosphorylated eukaryotic initiation factor-2α (p-eIF2α) and ATF4) were higher in NaF-treated cells.
Conclusion:A low concentration of NaF promotes the osteogenic/odontogenic differentiation of hDPCs and increases the level of ER stress.
- Full text:低浓度氟化钠对人牙髓细胞的成骨_成牙本质分化的影响.pdf
