Transcriptome Sequencing on Treatment of Kidney Deficiency and Blood Stasis-thin Endometrium in Rats with Bushen Huoxue Prescription Through Enema
10.13422/j.cnki.syfjx.20231311
- VernacularTitle:补肾活血方导法治疗薄型子宫内膜-肾虚血瘀证模型大鼠的全转录测序分析
- Author:
Xuan ZHANG
1
;
Wanting XIA
1
;
Zhixing YIN
1
;
Nana HAN
1
;
Jinzhu HUANG
2
;
Hang ZHOU
3
;
Yi WANG
1
;
Juan LI
1
;
Qian ZENG
1
Author Information
1. Hospital of Chengdu University of Traditional Chinese Medicine （TCM）， Chengdu 610075， China
2. School of Nursing， Chengdu University of TCM， Chengdu 610075， China
3. School of Basic Medical Sciences， Chengdu University of TCM， Chengdu 610075， China
- Publication Type:Journal Article
- Keywords:
thin endometrium;
syndrome of kidney deficiency and blood stasis;
Bushen Huoxue enema intervention;
transcriptome sequencing;
disease-related specific competitive endogenous RNA（ceRNA） regulatory network
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(2):79-90
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the mechanism of Bushen Huoxue enema in treating the rat model of kidney deficiency and blood stasis-thin endometrium （KDBS-TE） by transcriptome sequencing. MethodThe rat model of KDBS-TE was established by administration of tripterygium polyglycosides tablets combined with subcutaneous injection of adrenaline. The pathological changes of rat endometrium in each group were then observed. Three uterine tissue specimens from each of the blank group， model group， and Bushen Huoxue enema group were randomly selected for transcriptome sequencing. The differentially expressed circRNAs， lncRNAs， and miRNAs were screened， and the disease-related specific competitive endogenous RNA （ceRNA） regulatory network was constructed. Furthermore， the gene ontology （GO） functional annotation and the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment were performed for the mRNAs in the network. ResultCompared with the blank group， the model group showed endometrial dysplasia， decreased endometrial thickness and endometrial/total uterine wall thickness ratio （P<0.01）， and differential expression of 18 circRNAs， 410 lncRNAs， and 7 miRNAs. Compared with the model group， the enema and estradiol valerate groups showed improved endometrial morphology and increased endometrial thickness and ratio of endometrial to total uterine wall thickness （P<0.05）. In addition， 21 circRNAs， 518 lncRNAs， and 17 miRNAs were differentially expressed in the enema group. The disease-related specific circRNA-miRNA-mRNA regulatory network composed of 629 nodes and 664 edges contained 2 circRNAs， 34 miRNAs， and 593 mRNAs. The lncRNA-miRNA-mRNA regulatory network composed of 180 nodes and 212 edges contained 5 lncRNAs， 10 miRNAs， and 164 mRNAs. The mNRAs were mainly enriched in Hippo signaling pathway， autophagy-animal， axon guidance， etc. ConclusionBushen Huoxue enema can treat KDBS-TE in rats by regulating specific circRNAs， lncRNAs， and miRNAs in the uterus and the ceRNA network.
