The Effects of Desflurane on Ca2+ Release from the Myocardial Sarcoplasmic Reticulum in Vitro.
10.4097/kjae.2001.40.3.376
- Author:
Sun Joon BAI
1
;
Young Seok LEE
;
Jae Kwang SHIM
;
Jin Soo KIM
;
Jin Cheon MOON
;
Yong Taek NAM
Author Information
1. Department of Anesthesiology, Yonsei University College of Medicine Seoul.
- Publication Type:In Vitro ; Original Article
- Keywords:
Anesthetics, volatile;
desflurane;
Heart: action potential;
calcium current;
contractility;
rapid colling contracture
- MeSH:
Action Potentials;
Animals;
Contracture;
Depression;
Guinea Pigs;
Isoflurane;
Microelectrodes;
Myocardial Contraction;
Myocardium;
Papillary Muscles;
Rats;
Sarcoplasmic Reticulum*
- From:Korean Journal of Anesthesiology
2001;40(3):376-383
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The purpose of this study is to examine the effect of Desflurane on myocardial contractility and cellular electrophysiologic behabior in isolated guinea pig and rat right ventricular papillary muscle. METHODS: The isometric force of a guinea pig ventricular papillary muscle was studied in normal and 26 mM Tyrode's solution at various stimulation rates. Experiments using rat papillary muscles under normal Tyrode's solution at resting-state (RS) and using guinea pig papillary muscles under low Na Tyrode's solution (25 mM) were performed to evaluate the effect on Ca2+ release from the sarcoplasmic reticulum (SR). Normal and slow action potentials (APs) were evaluated by using a conventional microelectrode technique. Effects of desflurane on SR function in situ were examined by its effect on rapid colling contractures (RCCs). 1 MAC (end-tidal concentration: 6%) and 2 MAC desflurane were applied. RESULTS: Desflurane equivalent to 6% and 12% depressed guinea pig myocardial contractions in the control to -70% and -40% from RS to 3 Hz stimulation rates. Contractile force after rest in rat and guinea pig myocardium under low Na Tyrode's solution showed modest depression. In the partially depolarized, beta-adrenergically stimulated myocardium, 6% and 12% desflurane caused marked depression of late force (6%: -60%, 12%: -80%) with moderate changes of early peak force (6%: -20%, 12%: -40%). RCCs were abolished at 6% concentration. CONCLUSIONS: The direct myocardial depressant effects of desflurane is slightly greater to those seen with isoflurane. The rapid initial release of Ca2+ from the SR by depolarization seems to be modestly depressed, although certain release pathways induced by rapid colling appear to be markedly depressed.
