Effects of Pogonatherum paniceum (Lamk) Hack extract on antimitochondrial DNA mediated inflammation by attenuating Tlr9 expression in LPS-induced macrophages
- Author:
Rungthip THONGBOONTHO
1
;
Kanoktip PETCHARAT
;
Narongsuk MUNKONG
;
Chakkraphong KHONTHUN
;
Atirada BOONDECH
;
Kanokkarn PHROMNOI
;
Arthid THIM-UAM
Author Information
- Publication Type:Original Research
- From:Nutrition Research and Practice 2023;17(5):827-843
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES:Mitochondrial DNA leakage leads to inflammatory responses via endosome activation. This study aims to evaluate whether the perennial grass water extract (Pogonatherum paniceum) ameliorate mitochondrial DNA (mtDNA) leakage.MATERIALS/METHODS: The major bioactive constituents of P. paniceum (PPW) were investigated by high-performance liquid chromatography, after which their antioxidant activities were assessed. In addition, RAW 264.7 macrophages were stimulated with lipopolysaccharide, resulting in mitochondrial damage. Quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were used to examine the gene expression and cytokines.
RESULTS:Our results showed that PPW extract-treated activated cells significantly decrease reactive oxygen species and nitric oxide levels by reducing the p22 phox and iNOS expression and lowering cytokine-encoding genes, including IL-6, TNF-α, IL-1β, PG-E2 and IFN-γ relative to the lipopolysaccharide (LPS)-activated macrophages. Furthermore, we observed that LPS enhanced the mtDNA leaked into the cytoplasm, increasing the transcription of Tlr9 and signaling both MyD88/Irf7-dependent interferon and MyD88/NF-κb p65-dependent inflammatory cytokine mRNA expression but which was alleviated in the presence of PPW extract.
CONCLUSIONS:Our data show that PPW extract has antioxidant and anti-inflammatory activities by facilitating mtDNA leakage and lowering the Tlr9 expression and signaling activation.
