Porcine epidemic diarrhea viruses from Vietnam: isolation, characterization, and neutralizing activity
- Author:
Van Tan DO
1
;
Quang Lam TRUONG
;
Hoai Thu DAO
;
Thi Lan NGUYEN
;
Minna SHIN
;
Kyeong Ryeol SHIN
;
Tae-Wook HAHN
Author Information
- Publication Type:Original Article
- From:Korean Journal of Veterinary Research 2023;63(2):e18-
- CountryRepublic of Korea
- Language:English
- Abstract: Porcine epidemic diarrhea (PED) is characterized by acute enteritis, watery diarrhea, weight loss, dehydration, and death, with high mortality in neonatal piglets. In this study, 3 virus isolates collected in Vietnam between 2016 and 2017 were propagated successfully in Vero cells at high virus titers. Sequence analysis of the full-length spike (S) gene showed that all 3 isolates belong to genogroup 2b, which is closely related to other prevalent Asian strains. A comparison of the amino acid sequence revealed a 98.19% to 99.13% homology with the Vietnam isolates circulating during 2013–2015, suggesting that field PED viruses (PEDVs) are evolving continuously. Experiments in animals showed that the antisera from guinea pigs immunized with the vaccine strain resulted in higher levels (5 log2) of neutralizing antibodies against the homologous strain and a relatively moderate level of neutralizing antibodies against the field isolates. This finding would be helpful in selecting a PEDV strain for vaccine development.
